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PMID:16633814

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Citation

Raab, S, Toth, Z, de Groot, C, Stamminger, T and Hoth, S (2006) ABA-responsive RNA-binding proteins are involved in chloroplast and stromule function in Arabidopsis seedlings. Planta 224:900-14

Abstract

The phytohormone abscisic acid (ABA) regulates essential growth and developmental processes in plants. Recently, RNA-binding proteins have been described as components of ABA signaling during germination. We have identified ten ABA-regulated RNA-binding proteins in Arabidopsis seedlings. Among those genes, AtCSP41B and cpRNP29 are highly expressed in seedlings. Using promoter:reporter gene analyses, we showed that both AtCSP41B and cpRNP29 were in particular expressed in photosynthetically active organs like green cotyledons, leaves, and petioles. The analysis of CFP-fusion proteins demonstrates that cpRNP29 localized to chloroplasts and AtCSP41B to chloroplasts and stromules. Whereas RNA-binding of cpRNP29 has previously been shown, we demonstrated through in vitro RNA-binding assays that recombinant AtCSP41B binds to RNA, and that chloroplast petD RNA can serve as a target of AtCSP41B. Developmental or environmental stimuli affected the expression of AtCSP41B and cpRNP29 in seedlings. Both genes were repressed during senescence, but only AtCSP41B was significantly repressed upon water stress. In addition, AtCSP41B and cpRNP29 exhibited low expression in etiolated seedlings compared to green seedlings, and cpRNP29 was regulated during the day photoperiod. Homozygous T-DNA insertion lines were isolated, characterized on the molecular level, and monitored for phenotypic changes. Taken together, the data show that both proteins are regulated during processes that are known to involve ABA signaling. Their localization in chloroplasts and RNA-binding activity suggest a role in chloroplast RNA metabolism in Arabidopsis seedlings.

Links

PubMed Online version:10.1007/s00425-006-0282-4

Keywords

Abscisic Acid/physiology; Aging/metabolism; Arabidopsis/physiology; Chloroplasts/physiology; DNA, Bacterial; DNA, Chloroplast/metabolism; Gene Expression Regulation, Plant; Genes, Reporter; Mutagenesis, Insertional; Photoperiod; Plants, Genetically Modified; Promoter Regions, Genetic; RNA, Chloroplast/metabolism; RNA-Binding Proteins/physiology; Seedling/physiology; Water/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:CP41B

located_in

GO:0010319: stromule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:CP41B

involved_in

GO:0009611: response to wounding

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:CP41B

enables

GO:0003723: RNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:CP41B

involved_in

GO:0042631: cellular response to water deprivation

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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