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PMID:16624863

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Citation

Collier, S, Pendle, A, Boudonck, K, van Rij, T, Dolan, L and Shaw, P (2006) A distant coilin homologue is required for the formation of cajal bodies in Arabidopsis. Mol. Biol. Cell 17:2942-51

Abstract

Cajal bodies (CBs) are subnuclear bodies that are widespread in eukaryotes, being found in mammals, many other vertebrates and in all plant species so far examined. They are mobile structures, moving, fusing, and budding within the nucleus. Here we describe a screen for Arabidopsis mutants with altered CBs and describe mutants that have smaller Cajal bodies (ncb-2, ncb-3), lack them altogether (ncb-1), have increased numbers of CBs (pcb) or have flattened CBs (ccb). We have identified the gene affected in the ncb mutants as a distant homolog of the vertebrate gene that encodes coilin (At1g13030) and have termed the resulting protein Atcoilin. A T-DNA insertional mutant in this gene (ncb-4) also lacks Cajal bodies. Overexpression of Atcoilin cDNA in ncb-1 restores Cajal bodies, which recruit U2B as in the wild type, but which are, however, much larger than in the wild type. Thus we have shown that At1g13030 is required for Cajal body formation in Arabidopsis, and we hypothesize that the level of its expression is correlated with Cajal body size. The Atcoilin gene is unaffected in pcb and ccb, suggesting that other genes can also affect CBs.

Links

PubMed PMC1483031 Online version:10.1091/mbc.E05-12-1157

Keywords

Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/ultrastructure; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Cell Nucleus/metabolism; Cell Nucleus/ultrastructure; Coiled Bodies/genetics; Coiled Bodies/metabolism; Coiled Bodies/ultrastructure; DNA Mutational Analysis; Gene Expression; Genes, Plant/physiology; Molecular Sequence Data; Mutagenesis, Insertional; Nuclear Proteins/genetics; Nuclear Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:RU2B1

located_in

GO:0015030: Cajal body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:RU2B1

NOT|located_in

GO:0005730: nucleolus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:RU2B1

located_in

GO:0005654: nucleoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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