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PMID:16604063

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Citation

Muñoz, R, Moreno, M, Oliva, C, Orbenes, C and Larraín, J (2006) Syndecan-4 regulates non-canonical Wnt signalling and is essential for convergent and extension movements in Xenopus embryos. Nat. Cell Biol. 8:492-500

Abstract

Early shaping of Xenopus laevis embryos occurs through convergent and extension movements, a process that is driven by intercalation of polarized dorsal mesodermal cells and regulated by non-canonical Wnt signalling. Here, we have identified Xenopus syndecan-4 (xSyn4), a cell-surface transmembrane heparan sulphate proteoglycan. At the gastrula stage, xSyn4 is expressed in the involuting dorsal mesoderm and the anterior neuroectoderm. Later, it is found in the pronephros, branchial arches, brain and tailbud. Both gain- and loss-of-function of xSyn4 impaired convergent extension movements in Xenopus embryos and in activin-treated ectodermal explants. xSyn4 interacts functionally and biochemically with the Wnt receptor Frizzled7 (xFz7) and its signal transducer Dishevelled (xDsh). Furthermore, xSyn4 is necessary and sufficient for translocation of xDsh to the plasma membrane - a landmark in the activation of non-canonical Wnt signalling. Our results suggest that the ability of xSyn4 to translocate xDsh is regulated by fibronectin, a component of the extracellular matrix required for proper convergent extension movements. We propose a model where xSyn4 and fibronectin cooperate with xFz7 and Wnt in the specific activation of the non-canonical Wnt pathway.

Links

PubMed Online version:10.1038/ncb1399

Keywords

Adaptor Proteins, Signal Transducing/metabolism; Amino Acid Sequence; Animals; Cell Movement; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Fibronectins/metabolism; Gene Expression Regulation, Developmental; Membrane Glycoproteins/chemistry; Membrane Glycoproteins/genetics; Membrane Glycoproteins/metabolism; Models, Biological; Molecular Sequence Data; Phosphoproteins/metabolism; Phylogeny; Protein Binding; Proteoglycans/chemistry; Proteoglycans/genetics; Proteoglycans/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Receptors, G-Protein-Coupled/metabolism; Signal Transduction; Syndecan-4; Wnt Proteins/metabolism; Xenopus Proteins/metabolism; Xenopus laevis/embryology; Xenopus laevis/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

XENLA:DVL2

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

XENLA:DVL2

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

XENLA:DVL2

enables

GO:0045545: syndecan binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q1AGV6

F

Seeded From UniProt

complete

XENLA:DVL2

enables

GO:0045545: syndecan binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q1AGV7

F

Seeded From UniProt

complete

XENLA:SDC4B

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P51142

F

Seeded From UniProt

complete

XENLA:SDC4B

located_in

GO:0016021: integral component of membrane

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

XENLA:SDC4B

involved_in

GO:0008104: protein localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:SDC4B

involved_in

GO:0016055: Wnt signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9PUK8

P

Seeded From UniProt

complete

XENLA:SDC4B

involved_in

GO:0016055: Wnt signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P51142

P

Seeded From UniProt

complete

XENLA:SDC4B

enables

GO:0005109: frizzled binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9PUK8

F

Seeded From UniProt

complete

XENLA:SDC4B

involved_in

GO:0060027: convergent extension involved in gastrulation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:SDC4A

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P51142

F

Seeded From UniProt

complete

XENLA:SDC4A

located_in

GO:0016021: integral component of membrane

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

XENLA:SDC4A

involved_in

GO:0008104: protein localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:SDC4A

involved_in

GO:0016055: Wnt signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P51142

P

Seeded From UniProt

complete

XENLA:SDC4A

involved_in

GO:0016055: Wnt signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9PUK8

P

Seeded From UniProt

complete

XENLA:SDC4A

enables

GO:0005109: frizzled binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9PUK8

F

Seeded From UniProt

complete

XENLA:SDC4A

involved_in

GO:0060027: convergent extension involved in gastrulation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:FZD7A

involved_in

GO:0016055: Wnt signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

XENLA:FZD7A

enables

GO:0045545: syndecan binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q1AGV7

F

Seeded From UniProt

complete

XENLA:FZD7A

enables

GO:0045545: syndecan binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q1AGV6

F

Seeded From UniProt

complete

XENLA:FZD7A

involved_in

GO:0060027: convergent extension involved in gastrulation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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