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PMID:16595675

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Citation

Xiao, J, Dai, R, Negyessy, L and Bergson, C (2006) Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis. J. Biol. Chem. 281:15182-93

Abstract

In the central nervous system, clathrin-mediated endocytosis is crucial for efficient synaptic transmission. Clathrin-coated vesicle assembly and disassembly is regulated by some 30 adaptor and accessory proteins, most of which interact with clathrin heavy chain. Using the calcyon cytosolic domain as bait, we isolated clathrin light chain in a yeast two-hybrid screen. The interaction domain was mapped to the heavy chain binding domain and C-terminal regions of light chain. Further, the addition of the calcyon C terminus stimulated clathrin self-assembly in a dose-dependent fashion. Calcyon, which is a single transmembrane protein predominantly expressed in brain, localized to vesicular compartments within pre- and postsynaptic structures. There was a high degree of overlap in the distribution of LC and calcyon in neuronal dendrites, spines, and cell bodies. Co-immunoprecipitation studies further suggested an association of calcyon with the clathrin-mediated endocytic machinery. Compared with controls, HEK293 cells overexpressing calcyon exhibited significantly enhanced transferrin uptake but equivalent levels of recycling. Conversely, transferrin uptake was largely abolished in neocortical neurons obtained from mice homozygous for a calcyon null allele, whereas recycling proceeded at wild type levels. Collectively, these data indicate a role for calcyon in clathrin-mediated endocytosis in brain.

Links

PubMed Online version:10.1074/jbc.M600265200

Keywords

Animals; Base Sequence; Binding Sites; Cell Line; Cells, Cultured; Central Nervous System/physiology; Clathrin Light Chains/chemistry; Clathrin Light Chains/genetics; Clathrin Light Chains/metabolism; Endocytosis/physiology; Humans; In Vitro Techniques; Macaca mulatta; Membrane Proteins/chemistry; Membrane Proteins/deficiency; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Mice, Knockout; Microscopy, Electron; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurons/metabolism; Prefrontal Cortex/metabolism; Prefrontal Cortex/ultrastructure; Protein Structure, Tertiary; Rats; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Synaptic Transmission/physiology; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MACMU:F6VEL3

part_of

GO:0098793: presynapse

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0001950)

Seeded From UniProt

complete

HUMAN:CLCA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9NYX4

F

Seeded From UniProt

complete

RAT:CALY

involved_in

GO:1905445: positive regulation of clathrin coat assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CALY

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P09496

F

Seeded From UniProt

complete

HUMAN:CALY

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CALY

part_of

GO:0098843: postsynaptic endocytic zone

ECO:0005589: confocal microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0001950)

Seeded From UniProt

complete

HUMAN:CALY

part_of

GO:0098843: postsynaptic endocytic zone

ECO:0006003: electron microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0001950)

Seeded From UniProt

complete

HUMAN:CALY

part_of

GO:0098843: postsynaptic endocytic zone

ECO:0006063: over expression analysis evidence used in manual assertion

C

  • part_of:(UBERON:0001950)

Seeded From UniProt

complete

HUMAN:CALY

part_of

GO:0098843: postsynaptic endocytic zone

ECO:0005589: confocal microscopy evidence used in manual assertion

C

part_of:(UBERON:0001950)

Seeded From UniProt

complete

HUMAN:CALY

part_of

GO:0098843: postsynaptic endocytic zone

ECO:0006003: electron microscopy evidence used in manual assertion

C

part_of:(UBERON:0001950)

Seeded From UniProt

complete

HUMAN:CALY

part_of

GO:0098843: postsynaptic endocytic zone

ECO:0006063: over expression analysis evidence used in manual assertion

C

part_of:(UBERON:0001950)

Seeded From UniProt

complete

HUMAN:CALY

part_of

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CALY

enables

GO:0032051: clathrin light chain binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:CALY

involved_in

GO:0048268: clathrin coat assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CALY

involved_in

GO:0045807: positive regulation of endocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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