GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16579988

From GONUTS
Jump to: navigation, search
Citation

Kim, HS, Kim, EM, Lee, J, Yang, WH, Park, TY, Kim, YM and Cho, JW (2006) Heat shock protein 60 modified with O-linked N-acetylglucosamine is involved in pancreatic beta-cell death under hyperglycemic conditions. FEBS Lett. 580:2311-6

Abstract

The objective of this study was to identify proteins modified with O-linked N-acetylglucosamine (O-GlcNAc) in pancreatic beta-cells and to understand their roles in cell death under hyperglycemic conditions. Here we report that heat shock protein 60 (HSP60) is modified with O-GlcNAc. Levels of O-GlcNAcylated HSP60 increased twofold in response to hyperglycemic conditions. HSP60 is a chaperonin known to bind to Bax in the cytoplasm under normoglycemic conditions. Under hyperglycemic conditions, Bax detached from O-GlcNAcylated HSP60 and translocated to mitochondria. Hyperglycemic conditions were also associated with cytochrome c release, caspase-3 activation, and cell death, suggesting that elevated O-GlcNAcylation of HSP60 interferes with HSP60-Bax interactions, leading to pancreatic beta-cell death.

Links

PubMed Online version:10.1016/j.febslet.2006.03.043

Keywords

Acetylglucosamine/metabolism; Animals; Caspase 3; Caspases/metabolism; Cell Death; Cell Line; Chaperonin 60/metabolism; Cytochromes c/metabolism; Cytoplasm/metabolism; Hyperglycemia/metabolism; Insulin-Secreting Cells/metabolism; Mitochondria/metabolism; Protein Binding; Protein Processing, Post-Translational; Protein Transport; Rats; bcl-2-Associated X Protein/metabolism

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.