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PMID:16569675

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Citation

Caminschi, I, Corbett, AJ, Zahra, C, Lahoud, M, Lucas, KM, Sofi, M, Vremec, D, Gramberg, T, Pöhlmann, S, Curtis, J, Handman, E, van Dommelen, SL, Fleming, P, Degli-Esposti, MA, Shortman, K and Wright, MD (2006) Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN. Int. Immunol. 18:741-53

Abstract

CIRE/mDC-SIGN is a C-type lectin we originally identified as a molecule differentially expressed by mouse dendritic cell (DC) populations. Immunostaining with a CIRE/mDC-SIGN-specific mAb revealed that CIRE/mDC-SIGN is indeed on the surface of some CD4+, CD4- 8- DCs and plasmacytoid pre-DCs, but not on CD8+ DCs. It has been proposed that CIRE/mDC-SIGN is the functional orthologue of human DC-SIGN (hDC-SIGN), a molecule that both enhances T cell responses and facilitates antigen uptake. We assessed if CIRE/mDC-SIGN and hDC-SIGN exhibit functional similarities. CIRE/mDC-SIGN is down-regulated upon activation, but unlike hDC-SIGN, incubation with IL-4 and IL-13 did not enhance CIRE/mDC-SIGN expression, indicating differences in gene regulation. Like hDC-SIGN, CIRE/mDC-SIGN bound mannosylated residues. However, we could detect no role for CIRE/mDC-SIGN in T cell-DC interactions and the protein did not bind to pathogens known to interact with hDC-SIGN, including Leishmania mexicana, cytomegalovirus, HIV and lentiviral particles bearing the Ebolavirus glycoprotein. The binding of CIRE/mDC-SIGN to hDC-SIGN ligands was not rescued when CIRE/mDC-SIGN was engineered to express the stalk region of hDC-SIGN. We conclude that there are significant differences in the fine specificity of the C-type lectin domains of hDC-SIGN and CIRE/mDC-SIGN and that these two molecules may not be functional orthologues.

Links

PubMed Online version:10.1093/intimm/dxl011

Keywords

Amino Acid Sequence; Animals; Antibodies, Monoclonal/immunology; CHO Cells; Cell Adhesion Molecules/biosynthesis; Cell Adhesion Molecules/immunology; Cell Adhesion Molecules/metabolism; Cricetinae; Dendritic Cells/immunology; Dendritic Cells/metabolism; Humans; Lectins, C-Type/biosynthesis; Lectins, C-Type/immunology; Lectins, C-Type/metabolism; Ligands; Mannose/metabolism; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Molecular Sequence Data; Protein Binding; Rats; Rats, Wistar; Receptors, Cell Surface/biosynthesis; Receptors, Cell Surface/immunology; Receptors, Cell Surface/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:C209A

located_in

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:C209A

enables

GO:0005537: mannose binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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