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PMID:16569666

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Citation

Tanentzapf, G, Martin-Bermudo, MD, Hicks, MS and Brown, NH (2006) Multiple factors contribute to integrin-talin interactions in vivo. J. Cell. Sci. 119:1632-44

Abstract

The cytoplasmic protein talin is an essential part of the integrin-cytoskeleton link. We characterized the interaction between integrin and two conserved regions of talin, the N-terminal ;head' domain and the C-terminus, which includes the I/LWEQ domain, within the living organism. Green-fluorescent-protein-tagged head and C-terminal domains were recruited to integrin adhesion sites. Both required integrins for recruitment, but the C-terminal domain also required endogenous talin, showing it was not recruited directly by integrins. We used chimeric transmembrane proteins containing the cytoplasmic domain of the integrin beta subunit to examine the integrin-talin head interaction. Monomeric chimeric proteins did not recruit talin head, whereas dimeric chimeras efficiently recruited it and caused a strong inhibition of integrin-mediated adhesion. These chimeras recruited surprisingly few integrin-associated proteins, indicating that recruitment of talin did not initiate a cascade of recruitment. Mutagenesis of the integrin cytoplasmic domain, within the chimera, showed the dominant-negative inhibition was not due to talin sequestration alone and that additional interactions are required.

Links

PubMed Online version:10.1242/jcs.02859

Keywords

Amino Acid Motifs; Amino Acid Sequence; Animals; Cell Adhesion Molecules/metabolism; Cell Membrane/metabolism; Cytoplasm/metabolism; Cytoskeleton/metabolism; Drosophila; Green Fluorescent Proteins/metabolism; Integrins/genetics; Integrins/metabolism; Inteins; Molecular Sequence Data; Muscle Contraction; Muscles/embryology; Muscles/physiology; Mutation; Protein Binding; Talin/genetics; Talin/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:M9NDM3

enables

GO:0005178: integrin binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0004657

F

Seeded From UniProt

complete

DROME:M9PBW9

enables

GO:0005178: integrin binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0004657

F

Seeded From UniProt

complete

DROME:M9PEJ8

enables

GO:0005178: integrin binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0004657

F

Seeded From UniProt

complete

DROME:M9PF06

enables

GO:0005178: integrin binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0004657

F

Seeded From UniProt

complete

DROME:ITBX

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0260442

F

Seeded From UniProt

complete

DROME:ITBX

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ITA2

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q960C2

enables

GO:0005178: integrin binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0004657

F

Seeded From UniProt

complete

DROME:Q9VSL8

enables

GO:0005178: integrin binding

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0004657

F

Seeded From UniProt

complete


See also

References

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