GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16515795

From GONUTS
Jump to: navigation, search
Citation

Matsuda, T, Fujio, Y, Nariai, T, Ito, T, Yamane, M, Takatani, T, Takahashi, K and Azuma, J (2006) N-cadherin signals through Rac1 determine the localization of connexin 43 in cardiac myocytes. J. Mol. Cell. Cardiol. 40:495-502

Abstract

It has been proposed that the formation of gap junction is influenced by adherens junction in cardiac myocytes. To examine whether signals through N-cadherin are involved in the distribution of connexin 43 (Cx43), the distribution of cell-cell adhesion molecules, N-cadherin and Cx43, was analyzed in aligned cardiac myocytes. To induce cell orientation running in parallel to tension direction, neonatal rat cardiac myocytes were plated for 3 hours and exposed to 20% cyclic stretch for 24 hours on silicone dishes. The aligned cells cultured for 0-5 days were immunostained with anti- N-cadherin or anti-Cx43 antibody. After cultivation for 3-5 days, following the accumulation of N-cadherin, Cx43 was localized at the longitudinal cell termini. Adenoviral gene transfer of dominant negative N-cadherin significantly attenuated the localization of Cx43 at the longitudinal cell termini, suggesting that Cx43 localization is regulated downstream of N-cadherin. In the process of Cx43 localization, Rho family proteins, RhoA and Rac1, were activated, but not Cdc42. RhoA and Rac1 activation was inhibited by the transfection of dominant negative N-cadherin, indicating that RhoA and Rac1 were activated by N-cadherin in the oriented cardiac myocytes. The inhibition of Rho family proteins by Rho GDI significantly attenuated the accumulation of Cx43, but not that of N-cadherin. Furthermore, the translocation of Cx43 to longitudinal cell termini was prevented by the inhibition of Rac1, but not RhoA. Collectively, these findings suggest that the localization of Cx43 was determined through the Rac1 pathway downstream of N-cadherin in cardiac myocytes.

Links

PubMed Online version:10.1016/j.yjmcc.2005.12.010

Keywords

Animals; Animals, Newborn; Cadherins/genetics; Cadherins/metabolism; Cells, Cultured; Connexin 43/metabolism; Gap Junctions/metabolism; Gene Transfer Techniques; Myocardium/cytology; Myocardium/metabolism; Myocytes, Cardiac/cytology; Myocytes, Cardiac/metabolism; Protein Transport/physiology; Rats; Signal Transduction/physiology; rac1 GTP-Binding Protein/metabolism; rhoA GTP-Binding Protein

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:CXA1

located_in

GO:0005916: fascia adherens

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CXA1

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CADH2

located_in

GO:0005916: fascia adherens

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CADH2

involved_in

GO:0032880: regulation of protein localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:CADH2

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CADH2

involved_in

GO:0035023: regulation of Rho protein signal transduction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:CXA1

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CXA1

part_of

GO:0005916: fascia adherens

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.