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PMID:1651502
| Citation |
Dohmen, RJ, Madura, K, Bartel, B and Varshavsky, A (1991) The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme. Proc. Natl. Acad. Sci. U.S.A. 88:7351-5 |
|---|---|
| Abstract |
The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all organisms examined, from mammals to bacteria. We show that UBC2(RAD6), one of at least seven ubiquitin-conjugating enzymes in the yeast Saccharomyces cerevisiae, is essential for multiubiquitination and degradation of the N-end rule substrates. We also show that UBC2 is physically associated with UBR1, the recognition component of the N-end rule pathway. These results indicate that some of the UBC2 functions, which include DNA repair, induced mutagenesis, sporulation, and regulation of retrotransposition, are mediated by protein degradation via the N-end rule pathway. |
| Links | |
| Keywords |
DNA Repair; Fungal Proteins/metabolism; Haploidy; Ligases/genetics; Ligases/metabolism; Models, Biological; Protein Binding; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Ubiquitin-Conjugating Enzymes; Ubiquitins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
YEAST:UBC2 |
involved_in |
GO:0071596: ubiquitin-dependent protein catabolic process via the N-end rule pathway |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
YEAST:UBC2 |
involved_in |
GO:0000209: protein polyubiquitination |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
YEAST:UBC2 |
part_of |
GO:1990303: UBR1-RAD6 ubiquitin ligase complex |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P19812 |
C |
Seeded From UniProt |
complete | |
|
YEAST:UBC2 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P19812 |
F |
Seeded From UniProt |
complete | |
|
YEAST:UBR1 |
part_of |
GO:1990303: UBR1-RAD6 ubiquitin ligase complex |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P06104 |
C |
Seeded From UniProt |
complete | |
|
YEAST:UBR1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P06104 |
F |
Seeded From UniProt |
complete | |
See also
References
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