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PMID:16408306

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Citation

Baqri, R, Charan, R, Schimmelpfeng, K, Chavan, S and Ray, K (2006) Kinesin-2 differentially regulates the anterograde axonal transports of acetylcholinesterase and choline acetyltransferase in Drosophila. J. Neurobiol. 66:378-92

Abstract

Choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) are involved in acetylcholine synthesis and degradation at pre- and postsynaptic compartments, respectively. Here we show that their anterograde transport in Drosophila larval ganglion is microtubule-dependent and occurs in two different time profiles. AChE transport is constitutive while that of ChAT occurs in a brief pulse during third instar larva stage. Mutations in the kinesin-2 motor subunit Klp64D and separate siRNA-mediated knock-outs of all the three kinesin-2 subunits disrupt the ChAT and AChE transports, and these antigens accumulate in discrete nonoverlapping punctae in neuronal cell bodies and axons. Quantification analysis further showed that mutations in Klp64D could independently affect the anterograde transport of AChE even before that of ChAT. Finally, ChAT and AChE were coimmunoprecipitated with the kinesin-2 subunits but not with each other. Altogether, these suggest that kinesin-2 independently transports AChE and ChAT within the same axon. It also implies that cargo availability could regulate the rate and frequency of transports by kinesin motors.

Links

PubMed Online version:10.1002/neu.20230

Keywords

Acetylcholine/metabolism; Acetylcholinesterase/metabolism; Animals; Axonal Transport/genetics; Axons/metabolism; Central Nervous System/anatomy & histology; Central Nervous System/enzymology; Central Nervous System/growth & development; Choline O-Acetyltransferase/metabolism; Down-Regulation/genetics; Drosophila melanogaster/anatomy & histology; Drosophila melanogaster/enzymology; Drosophila melanogaster/growth & development; Ganglia, Invertebrate/cytology; Ganglia, Invertebrate/growth & development; Ganglia, Invertebrate/metabolism; Larva/cytology; Larva/genetics; Larva/metabolism; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Molecular Motor Proteins/genetics; Molecular Motor Proteins/metabolism; Mutation/genetics; Protein Subunits/genetics; Protein Subunits/metabolism; RNA Interference; Synapses/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:A8JUR4

involved_in

GO:0008089: anterograde axonal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:A8JUR5

involved_in

GO:0008089: anterograde axonal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:B7Z139

involved_in

GO:0008089: anterograde axonal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:M9PGW1

involved_in

GO:0008089: anterograde axonal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:VACHT

located_in

GO:0043195: terminal bouton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:CLAT

located_in

GO:0043195: terminal bouton

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:KLP68

involved_in

GO:0008089: anterograde axonal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q00085

involved_in

GO:0008089: anterograde axonal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VRK9

involved_in

GO:0008089: anterograde axonal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VZ07

involved_in

GO:0008089: anterograde axonal transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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