GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16394536

From GONUTS
Jump to: navigation, search
Citation

Choi, MH, Sun, HY, Park, RY, Bai, YH, Chung, YY, Kim, CM and Shin, SH (2006) Human serum albumin enhances the hemolytic activity of Vibrio vulnificus. Biol. Pharm. Bull. 29:180-2

Abstract

Vibrio vulnificus hemolysin (VvhA) is inactivated in the late growth phase by its oligomerization. Albumin is known to affect the activities of many bacterial toxins. In this study, we investigated the effects of human or bovine serum albumin (HSA or BSA) on the production and activity of VvhA. HSA did not affect V. vulnificus growth and vvhA transcription. However, VvhA hemolytic activity in culture supernatants was significantly higher in the presence of HSA than in the absence of HSA. By Western blot analysis, the oligomerization of VvhA was inhibited and the remaining active VvhA monomer was increased in culture supernatants containing HSA. BSA produced similar results. These findings indicate that both HSA and BSA stabilize VvhA and delay VvhA inactivation by oligomerization, and thus enhance VvhA activity.

Links

PubMed

Keywords

Animals; Bacterial Proteins/pharmacology; Blotting, Western; Cattle; Drug Synergism; Electrophoresis, Polyacrylamide Gel; Hemolysin Proteins; Hemolysis/drug effects; Humans; Serum Albumin/pharmacology; Serum Albumin, Bovine/pharmacology; Vibrio vulnificus/chemistry; beta-Galactosidase/blood

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

VIBVU:VLLY

involved_in

GO:0019836: hemolysis by symbiont of host erythrocytes

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ALBU

involved_in

GO:0032460: negative regulation of protein oligomerization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ALBU

enables

GO:0140272: exogenous protein binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.