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PMID:16341215

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Citation

Bermingham, JR Jr, Shearin, H, Pennington, J, O'Moore, J, Jaegle, M, Driegen, S, van Zon, A, Darbas, A, Ozkaynak, E, Ryu, EJ, Milbrandt, J and Meijer, D (2006) The claw paw mutation reveals a role for Lgi4 in peripheral nerve development. Nat. Neurosci. 9:76-84

Abstract

Peripheral nerve development results from multiple cellular interactions between axons, Schwann cells and the surrounding mesenchymal tissue. The delayed axonal sorting and hypomyelination throughout the peripheral nervous system of claw paw (clp) mutant mice suggest that the clp gene product is critical for these interactions. Here we identify the clp mutation as a 225-bp insertion in the Lgi4 gene. Lgi4 encodes a secreted and glycosylated leucine-rich repeat protein and is expressed in Schwann cells. The clp mutation affects Lgi4 mRNA splicing, resulting in a mutant protein that is retained in the cell. Additionally, siRNA-mediated downregulation of Lgi4 in wild-type neuron-Schwann cell cocultures inhibits myelination, whereas exogenous Lgi4 restores myelination in clp/clp cultures. Thus, the abnormalities observed in clp mice are attributable to the loss of Lgi4 function, and they identify Lgi4 as a new component of Schwann cell signaling pathway(s) that controls axon segregation and myelin formation.

Links

PubMed Online version:10.1038/nn1598

Keywords

Amino Acid Sequence; Animals; Axons/physiology; Base Sequence; Cloning, Molecular; Coculture Techniques; DNA Transposable Elements; DNA, Complementary/biosynthesis; DNA, Complementary/genetics; Down-Regulation/genetics; Down-Regulation/physiology; Foot Deformities/genetics; Gene Expression Regulation/genetics; Gene Expression Regulation/physiology; Genetic Complementation Test; Genotype; Immunohistochemistry; In Situ Hybridization; Lentivirus/genetics; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Data; Mutation/physiology; Myelin Sheath/physiology; Neurons, Afferent/physiology; Peripheral Nervous System/growth & development; Peripheral Nervous System/physiology; Phenotype; Proteins/genetics; Proteins/physiology; RNA, Small Interfering/genetics; Rats; Reverse Transcriptase Polymerase Chain Reaction; Schwann Cells/physiology; Transfection

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:LGI4

involved_in

GO:0031641: regulation of myelination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:LGI4

located_in

GO:0005615: extracellular space

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:LGI4

acts_upstream_of_or_within

GO:0042552: myelination

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:LGI4

acts_upstream_of_or_within

GO:0042552: myelination

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1856594

P

Seeded From UniProt

complete


See also

References

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