GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16332540

From GONUTS
Jump to: navigation, search
Citation

Horiuchi, D, Barkus, RV, Pilling, AD, Gassman, A and Saxton, WM (2005) APLIP1, a kinesin binding JIP-1/JNK scaffold protein, influences the axonal transport of both vesicles and mitochondria in Drosophila. Curr. Biol. 15:2137-41

Abstract

In a genetic screen for Kinesin heavy chain (Khc)-interacting proteins, we identified APLIP1, a neuronally expressed Drosophila homolog of JIP-1, a JNK scaffolding protein . JIP-1 and its homologs have been proposed to act as physical linkers between kinesin-1, which is a plus-end-directed microtubule motor, and certain anterograde vesicles in the axons of cultured neurons . Mutation of Aplip1 caused larval paralysis, axonal swellings, and reduced levels of both anterograde and retrograde vesicle transport, similar to the effects of kinesin-1 inhibition. In contrast, Aplip1 mutation caused a decrease only in retrograde transport of mitochondria, suggesting inhibition of the minus-end microtubule motor cytoplasmic dynein . Consistent with dynein defects, combining heterozygous mutations in Aplip1 and Dynein heavy chain (Dhc64C) generated synthetic axonal transport phenotypes. Thus, APLIP1 may be an important part of motor-cargo linkage complexes for both kinesin-1 and dynein. However, it is also worth considering that APLIP1 and its associated JNK signaling proteins could serve as an important signaling module for regulating transport by the two opposing motors.

Links

PubMed PMC1532932 Online version:10.1016/j.cub.2005.10.047

Keywords

Adaptor Proteins, Signal Transducing/metabolism; Amino Acid Sequence; Animals; Base Sequence; Biological Transport; Chromosome Mapping; Drosophila/physiology; Drosophila Proteins/physiology; Dyneins/metabolism; Kinesin/metabolism; Larva/physiology; Microtubules/metabolism; Mitochondria/physiology; Molecular Sequence Data; Mutation/genetics; Neurons/metabolism; Sequence Analysis, DNA; Transport Vesicles/physiology

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

See Help:References for how to manage references in GONUTS.