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PMID:16325501

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Citation

Mendoza, M, Redemann, S and Brunner, D (2005) The fission yeast MO25 protein functions in polar growth and cell separation. Eur. J. Cell Biol. 84:915-26

Abstract

Proteins of the MO25 family are widely conserved but their function has not been characterized in detail. Human MO25 is a cofactor of LKB1, a conserved protein kinase with roles in cell polarity in nematodes, flies and mammalian cells. Furthermore, the budding yeast MO25 homologue, Hym1, is important for cell separation and morphogenesis. We have characterized Pmo25p, the MO25 homologue in the fission yeast Schizosaccharomyces pombe. Pmo25p is an essential protein required for polar growth; in its absence the actin cytoskeleton becomes depolarized and cells adopt a round morphology. In addition, pmo25 mutants are defective in cell separation. Both functions of Pmo25p appear to be mediated by the Orb6p-Mob2p kinase complex. Pmo25p shows no distinct localization during interphase, but it is recruited to one of the two spindle pole bodies during anaphase and to the division site during cytokinesis. The septation initiation network (SIN) regulates the localization of Pmo25p, suggesting that it regulates Pmo25p function during cell division.

Links

PubMed Online version:10.1016/j.ejcb.2005.09.013

Keywords

Anaphase/physiology; Cell Cycle Proteins/genetics; Cell Cycle Proteins/physiology; Cell Division/physiology; Cell Polarity; Cytokinesis/physiology; Cytoskeleton/physiology; Gene Expression Regulation, Fungal; Genes, Fungal; Genes, cdc/physiology; Interphase/physiology; Microscopy, Fluorescence; Movement; Mutation; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/physiology; Schizosaccharomyces/cytology; Schizosaccharomyces/physiology; Schizosaccharomyces pombe Proteins/genetics; Schizosaccharomyces pombe Proteins/physiology; Spindle Apparatus/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:ORB6

involved_in

GO:2000100: regulation of establishment or maintenance of bipolar cell polarity regulating cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:MOB2

located_in

GO:0005938: cell cortex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:CDC7

located_in

GO:0071958: new mitotic spindle pole body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:SPN4

located_in

GO:0005940: septin ring

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:PMO25

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:PMO25

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:PMO25

located_in

GO:0035839: non-growing cell tip

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:PMO25

located_in

GO:0071958: new mitotic spindle pole body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:PMO25

located_in

GO:0032153: cell division site

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:PMO25

involved_in

GO:0071574: protein localization to medial cortex

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(PomBase:SPCC970.04c)

Seeded From UniProt

complete

YEAST:HYM1

part_of

GO:0044732: mitotic spindle pole body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:HYM1

GO:0044732: mitotic spindle pole body

ECO:0000315:

C

Figure 1 A-B: Pmo25 knockout mutant results in oval-shaped cells when compared to wild type strain with intact Pmo25 which result in elongated cell shape. This means protein Pmo25p is essential for polarized growth.

complete
CACAO 10124

YEAST:HYM1

GO:0044732: mitotic spindle pole body

ECO:0000314:

C

Figure 2

complete
CACAO 10125

YEAST:MOB2

GO:0005938: cell cortex

ECO:0000315:

C

Fig. 4C: Mob2p localizes to cell cortex.

complete
CACAO 10130

SCHPO:PMO25

part_of

GO:0032153: cell division site

ECO:0000314: direct assay evidence used in manual assertion

C

exists_during:(GO:0000281)

Seeded From UniProt

complete

SCHPO:PMO25

part_of

GO:0035839: non-growing cell tip

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:PMO25

involved_in

GO:0071574: protein localization to medial cortex

ECO:0000315: mutant phenotype evidence used in manual assertion

P

has_input:(PomBase:SPCC970.04c)

Seeded From UniProt

complete

SCHPO:PMO25

part_of

GO:0071958: new mitotic spindle pole body

ECO:0000314: direct assay evidence used in manual assertion

C

exists_during:(GO:0000090)

Seeded From UniProt

complete

SCHPO:PMO25

involved_in

GO:2000100: regulation of establishment or maintenance of bipolar cell polarity regulating cell shape

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:PMO25

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

exists_during:(GO:0051329)

Seeded From UniProt

complete

SCHPO:PMO25

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

exists_during:(GO:0051329)

Seeded From UniProt

complete

See also

References

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