GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16319922

From GONUTS
Jump to: navigation, search
Citation

Lee, MH, Hook, B, Lamont, LB, Wickens, M and Kimble, J (2006) LIP-1 phosphatase controls the extent of germline proliferation in Caenorhabditis elegans. EMBO J. 25:88-96

Abstract

Caenorhabditis elegans germline cells are maintained in an undifferentiated and mitotically dividing state by Notch signaling and the FBF (for fem-3 binding factor) RNA-binding protein. Here, we report that the LIP-1 phosphatase, a proposed homolog of mitogen-activated protein (MAP) kinase phosphatases, is required for the normal extent of germline proliferation, and that lip-1 controls germline proliferation by regulating MAP kinase activity. In wild-type germ lines, LIP-1 protein is present in the proximal third of the mitotic region, consistent with its effect on germline proliferation. We provide evidence that lip-1 expression in the germline mitotic region is controlled by a combination of GLP-1/Notch signaling and FBF repression. Unexpectedly, FBF controls the accumulation of lip-1 mRNA, and therefore is likely to control its stability or 3'-end formation. In a sensitized mutant background, LIP-1 can function as a pivotal regulator of the decision between proliferation and differentiation. The control of germline proliferation by LIP-1 has intriguing parallels with the control of stem cells and progenitor cells in vertebrates.

Links

PubMed PMC1351240 Online version:10.1038/sj.emboj.7600901

Keywords

3' Untranslated Regions/metabolism; Animals; Antibodies/immunology; Caenorhabditis elegans/enzymology; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/immunology; Caenorhabditis elegans Proteins/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Proliferation; Chromatin Immunoprecipitation; DNA-Binding Proteins/immunology; Gene Expression Regulation, Developmental; Germ Cells/enzymology; Germ Cells/growth & development; Mitogen-Activated Protein Kinase 1; Mitosis; Promoter Regions, Genetic; Protein Tyrosine Phosphatases/genetics; Protein Tyrosine Phosphatases/metabolism; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; RNA, Messenger/metabolism; RNA-Binding Proteins/metabolism; Trans-Activators/immunology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:DUSL

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00001401
WB:WBGene00001402

F

Seeded From UniProt

complete

CAEEL:DUSL

involved_in

GO:0007281: germ cell development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:DUSL

involved_in

GO:0007219: Notch signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

WB:WBGene00004765

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.