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PMID:16314516

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Citation

Cao, Q, Huang, YS, Kan, MC and Richter, JD (2005) Amyloid precursor proteins anchor CPEB to membranes and promote polyadenylation-induced translation. Mol. Cell. Biol. 25:10930-9

Abstract

The cytoplasmic polyadenylation element (CPE) binding factor, CPEB, is a sequence-specific RNA binding protein that controls polyadenylation-induced translation in germ cells and at postsynaptic sites of neurons. A yeast two-hybrid screen with a mouse brain cDNA library identified the transmembrane amyloid precursor-like protein 1 (APLP1) as a CPEB-interacting factor. CPEB binds the small intracellular domain (ICD) of APLP1 and the related proteins APLP2 and APP. These proteins promote polyadenylation and translation by stimulating Aurora A catalyzed CPEB serine 174 phosphorylation. Surprisingly, CPEB, Maskin, CPSF, and several other factors involved in polyadenylation and translation and CPE-containing RNA are all detected on membranes by cell fractionation and immunoelectron microscopy. Moreover, most of the RNA that undergoes polyadenylation does so in membrane-containing fractions. These data demonstrate a link between cytoplasmic polyadenylation and membrane association and implicate APP family member proteins as anchors for localized mRNA polyadenylation and translation.

Links

PubMed PMC1316979 Online version:10.1128/MCB.25.24.10930-10939.2005

Keywords

Amyloid beta-Protein Precursor/analysis; Amyloid beta-Protein Precursor/metabolism; Animals; Cell Membrane/chemistry; Cell Membrane/metabolism; Cytoplasm/metabolism; Gene Library; Mice; Neurons/chemistry; Neurons/metabolism; Phosphorylation; Polyadenylation; Protein Biosynthesis; Protein-Serine-Threonine Kinases/metabolism; RNA, Messenger/metabolism; RNA-Binding Proteins/analysis; RNA-Binding Proteins/metabolism; Serine/metabolism; Two-Hybrid System Techniques

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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