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PMID:16306994

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Citation

Hüttelmaier, S, Zenklusen, D, Lederer, M, Dictenberg, J, Lorenz, M, Meng, X, Bassell, GJ, Condeelis, J and Singer, RH (2005) Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1. Nature 438:512-5

Abstract

Localization of beta-actin messenger RNA to sites of active actin polymerization modulates cell migration during embryogenesis, differentiation and possibly carcinogenesis. This localization requires the oncofetal protein ZBP1 (Zipcode binding protein 1), which binds to a conserved 54-nucleotide element in the 3'-untranslated region of the beta-actin mRNA known as the 'zipcode'. ZBP1 promotes translocation of the beta-actin transcript to actin-rich protrusions in primary fibroblasts and neurons. It is not known how the ZBP1-RNA complex achieves asymmetric protein sorting by localizing beta-actin mRNA. Here we show that chicken ZBP1 modulates the translation of beta-actin mRNA. ZBP1 associates with the beta-actin transcript in the nucleus and prevents premature translation in the cytoplasm by blocking translation initiation. Translation only occurs when the ZBP1-RNA complex reaches its destination at the periphery of the cell. At the endpoint of mRNA transport, the protein kinase Src promotes translation by phosphorylating a key tyrosine residue in ZBP1 that is required for binding to RNA. These sequential events provide both temporal and spatial control over beta-actin mRNA translation, which is important for cell migration and neurite outgrowth.

Links

PubMed Online version:10.1038/nature04115

Keywords

Actins/analysis; Actins/biosynthesis; Actins/genetics; Animals; Avian Proteins/genetics; Avian Proteins/metabolism; Cell Line; Cell Polarity; Chickens; DNA-Binding Proteins; Glycoproteins/genetics; Glycoproteins/metabolism; Humans; Molecular Sequence Data; Phosphorylation; Protein Biosynthesis; Proto-Oncogene Proteins pp60(c-src)/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:IF2B1

part_of

GO:0030175: filopodium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:IF2B1

part_of

GO:0030426: growth cone

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:IF2B1

involved_in

GO:0010976: positive regulation of neuron projection development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:IF2B1

enables

GO:0003729: mRNA binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:IF2B1

involved_in

GO:0017148: negative regulation of translation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:IF2B1

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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