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PMID:1630316

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Citation

Sugiura, A, Nakashima, K, Tanaka, K and Mizuno, T (1992) Clarification of the structural and functional features of the osmoregulated kdp operon of Escherichia coli. Mol. Microbiol. 6:1769-76

Abstract

Expression of the Escherichia coli kdpABC operon, which is responsible for a high-affinity potassium-uptake system, is regulated in response to a change in the medium osmolarity. In this study, we clarified the structure and function of the kdpABC promoter including its regulatory sequence at the molecular level. The canonical -35 and -10 regions determined for the promoter were not fully functional, i.e. in addition to them, a cis-acting sequence located upstream of the -35 region was essential for full activation of the promoter. This upstream sequence was demonstrated to be the target site for the trans-acting activator, KdpE.

Links

PubMed

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Biological Transport; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Molecular Sequence Data; Operon; Osmolar Concentration; Potassium/metabolism; Promoter Regions, Genetic; Regulatory Sequences, Nucleic Acid; Trans-Activators/genetics; Trans-Activators/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:KDPE

enables

GO:0000987: cis-regulatory region sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:KDPE

enables

GO:0000986: bacterial-type proximal promoter sequence-specific DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:KDPE

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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