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PMID:16267153

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Citation

Mahjoub, MR, Trapp, ML and Quarmby, LM (2005) NIMA-related kinases defective in murine models of polycystic kidney diseases localize to primary cilia and centrosomes. J. Am. Soc. Nephrol. 16:3485-9

Abstract

A key feature of the polycystic kidney diseases is aberrant cell proliferation, a consequence of dysfunctional ciliary signaling. The NIMA-related kinases (Nek) Nek1 and Nek8 carry the causal mutations of two of the eight established mouse models of polycystic kidneys. Nek proteins have roles in cell cycle and may contribute to coordinate regulation of cilia and cell-cycle progression. Herein is reported that in a mouse kidney epithelial cell line, mNek1 localizes to centrosomes in interphase and remains associated with the mitotic spindle pole during mitosis. In contrast, mNek8 localizes to the proximal region of the primary cilium and is not observed in dividing cells. Knockdown of mNek8 by siRNA does not affect ciliary assembly. Taken together with the phenotypes of the mutant mice, these data suggest that mNek1 and mNek8 provide links between cilia, centrosomes, and cell-cycle regulation.

Links

PubMed Online version:10.1681/ASN.2005080824

Keywords

Animals; Blotting, Western; Cell Cycle/physiology; Cell Cycle Proteins/metabolism; Cells, Cultured; Centrosome/enzymology; Centrosome/physiology; Cilia/physiology; Disease Models, Animal; Epithelial Cells/cytology; Epithelial Cells/enzymology; Fluorescent Antibody Technique; Kidney/cytology; Mice; Mitosis/physiology; Polycystic Kidney Diseases/enzymology; Polycystic Kidney Diseases/physiopathology; Protein-Serine-Threonine Kinases/metabolism; RNA, Small Interfering/analysis; Sensitivity and Specificity; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:NEK1

located_in

GO:0005813: centrosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:NEK8

located_in

GO:0005929: cilium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:NEK1

part_of

GO:0005813: centrosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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