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PMID:16260785

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Citation

Metz, J, Wächter, A, Schmidt, B, Bujnicki, JM and Schwappach, B (2006) The yeast Arr4p ATPase binds the chloride transporter Gef1p when copper is available in the cytosol. J. Biol. Chem. 281:410-7

Abstract

Cellular ion homeostasis involves communication between the cytosol and the luminal compartment of organelles. This is particularly critical for metal ions because of their toxic potential. We have identified the yeast homologue of the prokaryotic ArsA protein, the homodimeric ATPase Arr4p, as a protein that binds to the yeast intracellular CLC chloride-transport protein, Gef1p. We show that binding of Arr4p to the C terminus of Gef1p requires the presence of yeast cytosol and is sensitive to a highly specific copper chelator in vitro and in vivo. Copper alone can substitute for cytosol to support the interaction of Arr4p with the C terminus of Gef1p. The migration behavior of Arr4p in nonreducing gel electrophoresis correlates with cellular copper deficiency, repletion, or stress. Our homology model of Arr4p shows that the antimony (arsenic) metal binding site of ArsA is not conserved in Arr4p. The model suggests that a pair of cysteines, Cys285 and Cys288, is located in the interface of the Arr4p dimer. These residues are required for Arr4p homodimerization and for binding to the C terminus of Gef1p. Whereas both proteins are required for normal growth under iron-limiting conditions, they play opposite roles when copper and heat stress are combined in an alkaline environment. Under these conditions, deltagef1 cells grow much better than wild type yeast, whereas deltaarr4 cells are unable to grow. Comparison of the deltaarr4 with the deltaarr4deltagef1 strain suggests that Arr4p antagonizes the function of Gef1p.

Links

PubMed Online version:10.1074/jbc.M507481200

Keywords

Adenosine Triphosphatases/chemistry; Adenosine Triphosphatases/immunology; Adenosine Triphosphatases/metabolism; Animals; Antibodies; Arsenate Reductases; Arsenite Transporting ATPases; Chloride Channels/metabolism; Chlorides/metabolism; Copper/metabolism; Cytosol/metabolism; Dimerization; Guinea Pigs; Heat-Shock Response; Ion Pumps; Multienzyme Complexes; Protein Structure, Secondary; Protein Structure, Tertiary; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:SNF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P12904

F

Seeded From UniProt

complete

YEAST:AAKG

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P06782

F

Seeded From UniProt

complete

YEAST:GEF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q12154

F

Seeded From UniProt

complete

YEAST:GET3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P37020

F

Seeded From UniProt

complete

YEAST:GET3

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q12154

F

Seeded From UniProt

complete


See also

References

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