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PMID:16239147

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Citation

Kaksonen, M, Toret, CP and Drubin, DG (2005) A modular design for the clathrin- and actin-mediated endocytosis machinery. Cell 123:305-20

Abstract

Endocytosis depends on an extensive network of interacting proteins that execute a series of distinct subprocesses. Previously, we used live-cell imaging of six budding-yeast proteins to define a pathway for association of receptors, adaptors, and actin during endocytic internalization. Here, we analyzed the effects of 61 deletion mutants on the dynamics of this pathway, revealing functions for 15 proteins, and we analyzed the dynamics of 8 of these proteins. Our studies provide evidence for four protein modules that cooperate to drive coat formation, membrane invagination, actin-meshwork assembly, and vesicle scission during clathrin/actin-mediated endocytosis. We found that clathrin facilitates the initiation of endocytic-site assembly but is not needed for membrane invagination or vesicle formation. Finally, we present evidence that the actin-meshwork assembly that drives membrane invagination is nucleated proximally to the plasma membrane, opposite to the orientation observed for previously studied actin-assembly-driven motility processes.

Links

PubMed Online version:10.1016/j.cell.2005.09.024

Keywords

Actin Capping Proteins; Actins/metabolism; Biological Markers/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Membrane/metabolism; Clathrin/genetics; Clathrin/metabolism; Clathrin-Coated Vesicles/metabolism; Cytoskeletal Proteins/genetics; Cytoskeletal Proteins/metabolism; Endocytosis; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Deletion; Green Fluorescent Proteins/metabolism; Kinetics; Luminescent Proteins/metabolism; Membrane Glycoproteins/genetics; Membrane Glycoproteins/metabolism; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Models, Biological; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:RV161

located_in

GO:0030479: actin cortical patch

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:RV167

located_in

GO:0030479: actin cortical patch

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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