PMID:16198122

Citation	Gounko, NV, Rybakin, V, Kalicharan, D, Siskova, Z, Gramsbergen, A and van der Want, JJ (2005) CRF and urocortin differentially modulate GluRdelta2 expression and distribution in parallel fiber-Purkinje cell synapses. Mol. Cell. Neurosci. 30:513-22
Abstract	Corticotropin-releasing factor (CRF) and urocortin (UCN) are closely related multifunctional regulators, governing, among other processes, Purkinje cell development. Here, we investigate the effects of CRF and UCN on Purkinje cells in organotypic slices. We show that both peptides upregulate delta2 ionotropic glutamate receptor gene expression, and increase the abundance of the receptor in the postsynaptic density. However, only UCN treatment results in increased delta2 protein level per Purkinje cell, implying the existence of posttranscriptional regulation of GluRdelta2 mRNA. CRF, in contrast, reduces the number of delta2-positive dendritic shafts per cell, implying that the increase of GluRdelta2 in remaining synapses may be mainly due to its retargeting. We further observed different patterns of GluRdelta2 distribution in the zone of postsynaptic density upon CRF and UCN treatment. CRF treatment results in a clustered distribution of GluRdelta2 along the postsynaptic density, whereas UCN treatment provides a linear distribution.
Links	PubMed Online version:10.1016/j.mcn.2005.08.013
Keywords	Animals; Cerebellar Cortex/drug effects; Cerebellar Cortex/metabolism; Cerebellar Cortex/ultrastructure; Corticotropin-Releasing Hormone/metabolism; Corticotropin-Releasing Hormone/pharmacology; Dendritic Spines/drug effects; Dendritic Spines/metabolism; Dendritic Spines/ultrastructure; Gene Expression Regulation/drug effects; Gene Expression Regulation/physiology; Glutamic Acid/metabolism; Neuronal Plasticity/drug effects; Neuronal Plasticity/physiology; Organ Culture Techniques; Presynaptic Terminals/drug effects; Presynaptic Terminals/metabolism; Presynaptic Terminals/ultrastructure; Protein Processing, Post-Translational/drug effects; Protein Processing, Post-Translational/physiology; Purkinje Cells/drug effects; Purkinje Cells/metabolism; Purkinje Cells/ultrastructure; RNA, Messenger/drug effects; RNA, Messenger/metabolism; Rats; Receptors, Glutamate/genetics; Signal Transduction/drug effects; Signal Transduction/physiology; Synaptic Membranes/drug effects; Synaptic Membranes/metabolism; Synaptic Transmission/drug effects; Synaptic Transmission/physiology; Up-Regulation/drug effects; Up-Regulation/physiology; Urocortins
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:GRID2	part_of	GO:0014069: postsynaptic density	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.