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PMID:16194878

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Citation

Zhang, WV and Stott, NS BMP-2-modulated chondrogenic differentiation in vitro involves down-regulation of membrane-bound beta-catenin. Cell Commun. Adhes. 11:89-102

Abstract

Bone morphogenetic proteins (BMPs) are important regulators of cellular differentiation and embryonic development. Beta catenin mediated nuclear signaling has been implicated in BMP-2-modulated chondrogenic differentiation in the pluripotential stem cell line C3H10T1/2. However, there is little information on the functional role of beta catenin in BMP-2-modulated differentiation of primary nontransformed mesenchymal cells. Here, we present evidence to show that BMP-2-induced chondrogenic differentiation in high-density primary mesenchymal culture is associated with a significant decrease in membrane-bound beta catenin by 72 hours compared to controls. Nuclear localization of beta catenin is not detectable by immunofluorescence and the TCF-responsive reporter vector TOPFLASH shows only background activity during chondrogenic differentiation. BMP-2-treated cultures show reduced cell-cell adhesion by 72 hours, which correlates with the changes in levels of membrane-bound beta catenin. Up-regulation of membrane-bound beta catenin blocks the effect of BMP-2 on both chondrogenic differentiation and cell-cell adhesiveness. These findings suggest that BMP-2 can modulate the adhesivity of adherens junctions through regulation of membrane bound beta catenin.

Links

PubMed Online version:10.1080/15419060490951790

Keywords

Animals; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins/pharmacology; Cadherins/genetics; Cell Adhesion; Cell Culture Techniques; Cell Differentiation/drug effects; Cell Differentiation/physiology; Cell Membrane/physiology; Chick Embryo; Chondrocytes/cytology; Chondrocytes/drug effects; Chondrocytes/physiology; Cytoskeletal Proteins/genetics; Gene Expression Regulation; Genes, Reporter; Limb Buds; Lithium Chloride/pharmacology; Mesoderm/cytology; Trans-Activators/genetics; Transforming Growth Factor beta/pharmacology; beta Catenin

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:O42486

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:O42486

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:CADH2

involved_in

GO:0001502: cartilage condensation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP2

involved_in

GO:0030509: BMP signaling pathway

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP2

involved_in

GO:0002062: chondrocyte differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:BMP2

involved_in

GO:0051042: negative regulation of calcium-independent cell-cell adhesion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:CADH2

involved_in

GO:0001502: cartilage condensation

ECO:0000304: author statement supported by traceable reference used in manual assertion


P

Seeded From UniProt

complete

CHICK:O42486

part_of

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CHICK:O42486

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:WNT1

involved_in

GO:0030514: negative regulation of BMP signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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