GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16193045

From GONUTS
Jump to: navigation, search
Citation

Ueguchi-Tanaka, M, Ashikari, M, Nakajima, M, Itoh, H, Katoh, E, Kobayashi, M, Chow, TY, Hsing, YI, Kitano, H, Yamaguchi, I and Matsuoka, M (2005) GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature 437:693-8

Abstract

Gibberellins (GAs) are phytohormones that are essential for many developmental processes in plants. It has been postulated that plants have both membrane-bound and soluble GA receptors; however, no GA receptors have yet been identified. Here we report the isolation and characterization of a new GA-insensitive dwarf mutant of rice, gid1. The GID1 gene encodes an unknown protein with similarity to the hormone-sensitive lipases, and we observed preferential localization of a GID1-green fluorescent protein (GFP) signal in nuclei. Recombinant glutathione S-transferase (GST)-GID1 had a high affinity only for biologically active GAs, whereas mutated GST-GID1 corresponding to three gid1 alleles had no GA-binding affinity. The dissociation constant for GA4 was estimated to be around 10(-7) M, enough to account for the GA dependency of shoot elongation. Moreover, GID1 bound to SLR1, a rice DELLA protein, in a GA-dependent manner in yeast cells. GID1 overexpression resulted in a GA-hypersensitive phenotype. Together, our results indicate that GID1 is a soluble receptor mediating GA signalling in rice.

Links

PubMed Online version:10.1038/nature04028

Keywords

Amino Acid Sequence; Binding, Competitive; Cloning, Molecular; Epistasis, Genetic; Genes, Plant/genetics; Gibberellins/metabolism; Gibberellins/pharmacology; Molecular Sequence Data; Mutation/genetics; Oryza sativa/drug effects; Oryza sativa/genetics; Oryza sativa/metabolism; Phenotype; Plant Proteins/chemistry; Plant Proteins/genetics; Plant Proteins/metabolism; Protein Binding; Solubility; Sterol Esterase/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ORYSJ:GID1

located_in

GO:0005634: nucleus

ECO:0000305: curator inference used in manual assertion

C

Seeded From UniProt

complete

ORYSJ:GID1

involved_in

GO:0009739: response to gibberellin

ECO:0000305: curator inference used in manual assertion

P

Seeded From UniProt

complete

ORYSJ:GID1

enables

GO:0010331: gibberellin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ORYSJ:GID1

GO:0010331: gibberellin binding

ECO:0000314:

F

Figure 4

complete
CACAO 8238


See also

References

See Help:References for how to manage references in GONUTS.