GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:16186258
| Citation |
Olsen, O, Moore, KA, Fukata, M, Kazuta, T, Trinidad, JC, Kauer, FW, Streuli, M, Misawa, H, Burlingame, AL, Nicoll, RA and Bredt, DS (2005) Neurotransmitter release regulated by a MALS-liprin-alpha presynaptic complex. J. Cell Biol. 170:1127-34 |
|---|---|
| Abstract |
Synapses are highly specialized intercellular junctions organized by adhesive and scaffolding molecules that align presynaptic vesicular release with postsynaptic neurotransmitter receptors. The MALS/Veli-CASK-Mint-1 complex of PDZ proteins occurs on both sides of the synapse and has the potential to link transsynaptic adhesion molecules to the cytoskeleton. In this study, we purified the MALS protein complex from brain and found liprin-alpha as a major component. Liprin proteins organize the presynaptic active zone and regulate neurotransmitter release. Fittingly, mutant mice lacking all three MALS isoforms died perinatally with difficulty breathing and impaired excitatory synaptic transmission. Excitatory postsynaptic currents were dramatically reduced in autaptic cultures from MALS triple knockout mice due to a presynaptic deficit in vesicle cycling. These findings are consistent with a model whereby the MALS-CASK-liprin-alpha complex recruits components of the synaptic release machinery to adhesive proteins of the active zone. |
| Links |
PubMed PMC2171538 Online version:10.1083/jcb.200503011 |
| Keywords |
Adaptor Proteins, Signal Transducing/genetics; Adaptor Proteins, Signal Transducing/metabolism; Animals; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cells, Cultured; Female; Gene Targeting; Macromolecular Substances/metabolism; Male; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Mice, Knockout; Neurons/physiology; Neurotransmitter Agents/metabolism; Particle Size; Patch-Clamp Techniques; Presynaptic Terminals/metabolism; Proteomics; Synaptic Transmission/genetics; Two-Hybrid System Techniques; Vesicular Transport Proteins |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
MOUSE:APBA1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
PR:O88952 |
F |
Seeded From UniProt |
complete | |
|
MOUSE:B2RXQ2 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
PR:O88952 |
F |
Seeded From UniProt |
complete | |
|
MOUSE:B2RXW8 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
PR:O88952 |
F |
Seeded From UniProt |
complete | |
|
MOUSE:B8QI33 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
PR:O88952 |
F |
Seeded From UniProt |
complete | |
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
PR:O70589 |
F |
Seeded From UniProt |
complete | ||
|
located_in |
GO:0045202: synapse |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
MOUSE:LIN7B |
acts_upstream_of_or_within |
GO:0007269: neurotransmitter secretion |
ECO:0000316: genetic interaction evidence used in manual assertion |
MGI:MGI:1330839 |
P |
|
Seeded From UniProt |
complete |
|
MOUSE:LIN7C |
located_in |
GO:0045202: synapse |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | |
|
MOUSE:LIN7C |
acts_upstream_of_or_within |
GO:0007269: neurotransmitter secretion |
ECO:0000316: genetic interaction evidence used in manual assertion |
MGI:MGI:1330858 |
P |
|
Seeded From UniProt |
complete |
|
MOUSE:Q3UJC0 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
PR:O88952 |
F |
Seeded From UniProt |
complete | |
|
MOUSE:LIPA2 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
PR:Q8BSS9 |
F |
Seeded From UniProt |
complete | |
|
MOUSE:LIPA2 |
located_in |
GO:0045202: synapse |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | |
|
MOUSE:LIN7A |
acts_upstream_of_or_within |
GO:0007269: neurotransmitter secretion |
ECO:0000316: genetic interaction evidence used in manual assertion |
MGI:MGI:1330839 |
P |
Seeded From UniProt |
complete | |
|
MOUSE:S4R1D4 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
PR:O88952 |
F |
Seeded From UniProt |
complete | |
|
MOUSE:S4R1I7 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
PR:O88952 |
F |
Seeded From UniProt |
complete | |
|
part_of |
GO:0045202: synapse |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(EMAPA:16894) |
Seeded From UniProt |
complete | ||
See also
References
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