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PMID:16170306

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Citation

Pearson, NJ, Cullen, CF, Dzhindzhev, NS and Ohkura, H (2005) A pre-anaphase role for a Cks/Suc1 in acentrosomal spindle formation of Drosophila female meiosis. EMBO Rep. 6:1058-63

Abstract

Conventional centrosomes are absent from a female meiotic spindle in many animals. Instead, chromosomes drive spindle assembly, but the molecular mechanism of this acentrosomal spindle formation is not well understood. We have screened female sterile mutations for defects in acentrosomal spindle formation in Drosophila female meiosis. One of them, remnants (rem), disrupted bipolar spindle morphology and chromosome alignment in non-activated oocytes. We found that rem encodes a conserved subunit of Cdc2 (Cks30A). As Drosophila oocytes arrest in metaphase I, the defect represents a new Cks function before metaphase-anaphase transition. In addition, we found that the essential pole components, Msps and D-TACC, were often mislocalized to the equator, which may explain part of the spindle defect. We showed that the second cks gene cks85A, in contrast, has an important role in mitosis. In conclusion, this study describes a new pre-anaphase role for a Cks in acentrosomal meiotic spindle formation.

Links

PubMed PMC1371029 Online version:10.1038/sj.embor.7400529

Keywords

Amino Acid Sequence; Anaphase/physiology; Animals; Cell Line; Chromosomes/metabolism; Cyclin B/metabolism; Cyclin-Dependent Kinases/genetics; Cyclin-Dependent Kinases/metabolism; Drosophila/physiology; Drosophila Proteins/metabolism; Female; Gene Expression Regulation, Developmental; Meiosis/physiology; Metaphase/physiology; Microtubule-Associated Proteins/metabolism; Mitotic Spindle Apparatus/genetics; Mitotic Spindle Apparatus/metabolism; Molecular Sequence Data; Mutation; Oocytes/metabolism; Phenotype; RNA/metabolism; Sex Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:CKS1

involved_in

GO:0007144: female meiosis I

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:CKS1

involved_in

GO:0007057: spindle assembly involved in female meiosis I

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VHN1

involved_in

GO:0000278: mitotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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