GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16135809

From GONUTS
Jump to: navigation, search
Citation

Park, J, Seo, T, Kim, H and Choe, J (2005) Sumoylation of the novel protein hRIP{beta} is involved in replication protein A deposition in PML nuclear bodies. Mol. Cell. Biol. 25:8202-14

Abstract

Replication protein A (RPA) is a single-stranded-DNA-binding protein composed of three subunits with molecular masses of 70, 32, and 14 kDa. The protein is involved in multiple processes of eukaryotic DNA metabolism, including DNA replication, repair, and recombination. In Xenopus, Xenopus RPA-interacting protein alpha has been identified as a carrier molecule of RPA into the nucleus. In this study, human RPA-interacting protein alpha (hRIPalpha) and five novel splice isoforms (named hRIPalpha, hRIPbeta, hRIPgamma, hRIPdelta1, hRIPdelta2, and hRIPdelta3 according to the lengths of their encoding peptides) were cloned. Among hRIP isoforms, hRIPalpha and hRIPbeta were found to be the major splice isoforms and to show different subcellular localizations. While hRIPalpha localized to the cytoplasm, hRIPbeta was found in the PML nuclear body. Modification of hRIPbeta by sumoylation was found to be required for localization to the PML nuclear body. The results of the present work demonstrate that hRIPbeta transports RPA into the PML nuclear body and releases RPA upon UV irradiation. hRIPbeta thus plays an important role in RPA deposition in PML nuclear bodies and thereby supplements RPA for DNA metabolism.

Links

PubMed PMC1234305 Online version:10.1128/MCB.25.18.8202-8214.2005

Keywords

Active Transport, Cell Nucleus; Alternative Splicing; Amino Acid Sequence; Carrier Proteins/analysis; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Cycle/radiation effects; Cell Nucleus/chemistry; Cell Nucleus/metabolism; Cells, Cultured; Cloning, Molecular; Cytoplasm/chemistry; Cytoplasm/metabolism; DNA-Binding Proteins/analysis; DNA-Binding Proteins/metabolism; Humans; Molecular Sequence Data; Neoplasm Proteins/metabolism; Nuclear Localization Signals/genetics; Nuclear Localization Signals/metabolism; Nuclear Proteins/metabolism; Protein Isoforms/analysis; Protein Isoforms/genetics; Protein Isoforms/metabolism; Replication Protein A; S Phase/radiation effects; Transcription Factors/metabolism; Tumor Suppressor Proteins/metabolism; Ultraviolet Rays

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:RIP

involved_in

GO:0006606: protein import into nucleus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:RIP

involved_in

GO:0006310: DNA recombination

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P27694

P

Seeded From UniProt

complete

HUMAN:RIP

involved_in

GO:0006281: DNA repair

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P27694

P

Seeded From UniProt

complete

HUMAN:RIP

involved_in

GO:0006261: DNA-dependent DNA replication

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P27694

P

Seeded From UniProt

complete

HUMAN:RIP

enables

GO:0044877: protein-containing complex binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P27694

F

Seeded From UniProt

complete

HUMAN:RIP

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RIP

involved_in

GO:0009411: response to UV

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:RIP

part_of

GO:0016605: PML body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RFA1

part_of

GO:0016605: PML body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RFA1

part_of

GO:0005662: DNA replication factor A complex

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P15927

C

Seeded From UniProt

complete

HUMAN:RFA2

part_of

GO:0005662: DNA replication factor A complex

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P27694

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.