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PMID:16135223

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Citation

Cho, KH and Caparon, MG (2005) Patterns of virulence gene expression differ between biofilm and tissue communities of Streptococcus pyogenes. Mol. Microbiol. 57:1545-56

Abstract

The ability of Streptococcus pyogenes to form biofilm-like bacterial communities during infection of soft tissue has suggested that the capacity to produce biofilm may be important for pathogenesis. To examine this relationship, a panel of mutants was evaluated for their ability to form biofilm on abiotic surfaces in several assays. Several established virulence factors were crucial for biofilm formation, including the M protein, required for initial cell-surface interactions, and the hyaluronic acid capsule, required for subsequent maturation into a three-dimensional structure. Mutants lacking the transcription regulators Mga and CovR (CsrR) also failed to form biofilm. Comparison of transcriptional profiles revealed differential regulation of approximately 25% of the genome upon adaptation to biofilm. During infection of zebrafish, several virulence factors (notably cysteine protease and streptokinase) were regulated in a biofilm-like manner. However, the overall profile of virulence factor expression indicated that tissue communities have a pattern of gene expression different from biofilm. Taken together, these data show that while biofilm and tissue communities have many characteristics in common, that biofilm reproduces only a subset of the myriad cues used by tissue communities for regulation of virulence.

Links

PubMed Online version:10.1111/j.1365-2958.2005.04786.x

Keywords

Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biofilms/growth & development; Disease Models, Animal; Ecosystem; Gene Expression Regulation, Bacterial; Humans; Mutation; Oligonucleotide Array Sequence Analysis; Soft Tissue Infections/microbiology; Soft Tissue Infections/physiopathology; Streptococcal Infections/microbiology; Streptococcal Infections/physiopathology; Streptococcus pyogenes/genetics; Streptococcus pyogenes/growth & development; Streptococcus pyogenes/pathogenicity; Transcription, Genetic; Virulence; Zebrafish

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

STRPY:HASA

GO:0090609: single-species submerged biofilm formation

ECO:0000315:

P

Figure 5. shows that HasA mutants were unable to form biofilm.

complete
CACAO 5246

STRPY:HASA

involved_in

GO:0090609: single-species submerged biofilm formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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