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PMID:16112649

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Citation

Depping, R, Lohaus, C, Meyer, HE and Rüger, W (2005) The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: target proteins identified. Biochem. Biophys. Res. Commun. 335:1217-23

Abstract

Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total approximately 27) and ModB (total approximately 8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.

Links

PubMed Online version:10.1016/j.bbrc.2005.08.023

Keywords

ADP Ribose Transferases/metabolism; Bacteriophage T4/metabolism; Binding Sites; Drug Delivery Systems/methods; Escherichia coli/virology; Escherichia coli Proteins/metabolism; Peptide Elongation Factor Tu/metabolism; Peptidylprolyl Isomerase/metabolism; Protein Binding; Protein Interaction Mapping; Virus Replication/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPT4:MODB

enables

GO:0003956: NAD(P)+-protein-arginine ADP-ribosyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

BPT4:ALT

enables

GO:0003956: NAD(P)+-protein-arginine ADP-ribosyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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