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PMID:16098148
| Citation |
Watanabe-Fukunaga, R, Iida, S, Shimizu, Y, Nagata, S and Fukunaga, R (2005) SEI family of nuclear factors regulates p53-dependent transcriptional activation. Genes Cells 10:851-60 |
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| Abstract |
SEI family proteins, p34SEI-1 and SEI-2(TRIP-Br2), are nuclear factors that are implicated in cell cycle regulation through interaction with CDK4/CyclinD and E2F-1/DP-1 complexes. Here we report that the SEI family proteins regulate transcriptional activity of p53 tumor suppressor protein. Expression of SEI-1, SEI-2 or SEI-3 strongly stimulates p53-dependent gene activation in HeLa and U2OS cells but not in p53-deficient Saos2 or p53-knockdown HeLa cells. SEI proteins possess an intrinsic transactivation activity, interact with the coactivator CREB-binding protein, and cooperate synergistically with the ING family of chromatin-associated proteins to stimulate the transactivation function of p53. Doxycycline-induced expression of SEI proteins results in activation of the p21 gene and inhibition of cell growth, but the growth arrest was not suppressed by the siRNA-mediated knockdown of the endogenous p53 protein. These results indicate that the SEI family of nuclear proteins regulates p53 transcriptional activity and a p53-independent signaling pathway leading to growth inhibition. |
| Links |
PubMed Online version:10.1111/j.1365-2443.2005.00881.x |
| Keywords |
Adaptor Proteins, Signal Transducing; Amino Acid Motifs; Amino Acid Sequence; Cell Culture Techniques; Cell Cycle; Cell Cycle Proteins; Chromatin; HeLa Cells; Humans; Membrane Proteins/metabolism; Molecular Sequence Data; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Phosphoproteins/metabolism; RNA, Messenger/metabolism; Sequence Homology, Amino Acid; Signal Transduction; Species Specificity; Time Factors; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcriptional Activation; Tumor Suppressor Protein p53/metabolism; Two-Hybrid System Techniques |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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