GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:16055061

From GONUTS
Jump to: navigation, search
Citation

Verstreken, P, Ly, CV, Venken, KJ, Koh, TW, Zhou, Y and Bellen, HJ (2005) Synaptic mitochondria are critical for mobilization of reserve pool vesicles at Drosophila neuromuscular junctions. Neuron 47:365-78

Abstract

In a forward screen for genes affecting neurotransmission in Drosophila, we identified mutations in dynamin-related protein (drp1). DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses. Although resting Ca2+ is elevated at drp1 NMJs, basal synaptic properties are barely affected. However, during intense stimulation, mutants fail to maintain normal neurotransmission. Surprisingly, FM1-43 labeling indicates normal exo- and endocytosis, but a specific inability to mobilize reserve pool vesicles, which is partially rescued by exogenous ATP. Using a variety of drugs, we provide evidence that reserve pool recruitment depends on mitochondrial ATP production downstream of PKA signaling and that mitochondrial ATP limits myosin-propelled mobilization of reserve pool vesicles. Our data suggest a specific role for mitochondria in regulating synaptic strength.

Links

PubMed Online version:10.1016/j.neuron.2005.06.018

Keywords

Animals; Calcium/metabolism; Cyclic AMP-Dependent Protein Kinases/metabolism; Cytoskeletal Proteins; Drosophila/growth & development; Drosophila/metabolism; Drosophila/physiology; Drosophila/ultrastructure; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Electric Stimulation/methods; Endocytosis; GTP Phosphohydrolases/isolation & purification; GTP Phosphohydrolases/physiology; GTP-Binding Proteins; Larva; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mitochondria/metabolism; Mitochondria/physiology; Mitochondria/ultrastructure; Mutation; Myosin Light Chains/metabolism; Myosins/metabolism; Neuromuscular Junction/metabolism; Neuromuscular Junction/physiology; Neuromuscular Junction/ultrastructure; Neuropeptides/genetics; Neuropeptides/metabolism; Neurotransmitter Agents/metabolism; Presynaptic Terminals/metabolism; Synapses/metabolism; Synapses/physiology; Synaptic Transmission; Synaptic Vesicles/physiology; Tissue Distribution; Vesicular Transport Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q8IHG0

involved_in

GO:0051646: mitochondrion localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q8T9L0

involved_in

GO:0051646: mitochondrion localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VQE0

involved_in

GO:0051646: mitochondrion localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.