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PMID:16040607

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Citation

Ueki, T and Inouye, S (2005) Identification of a gene involved in polysaccharide export as a transcription target of FruA, an essential factor for Myxococcus xanthus development. J. Biol. Chem. 280:32279-84

Abstract

Fruiting body development in Myxococcus xanthus is a multicellular event that is coordinated by exchanging intercellular signals. FruA is a transcription factor essential for fruiting body development and is thought to play a key role in the C-signal pathway. Here we present the first identification of a gene regulated by FruA. The gene was isolated from a genomic library via in vitro selection in a DNA binding assay by using the DNA-binding domain of FruA tagged with His(8) at the C-terminal end (FruA-DBD-H(8)). The gene, named fdgA (FruA-dependent gene A), encodes a protein homologous to the outer-membrane auxiliary family protein involved in the polysaccharide export system. FruA-DBD-H(8) bound the upstream promoter region of the fdgA gene from nucleotide -89 to nucleotide -64 with respect to the transcription initiation site, which was required for the induction of fdgA expression during development. fdgA mRNA induced during development was absent in a fruA deletion strain. The deletion of fdgA resulted in defective fruiting body formation and reduced sporulation efficiency (1% that of the parent strain). Moreover, FruA was required for the developmental expression of sasA, which is also involved in the biosynthesis of the lipopolysaccharide O-antigen and is required for fruiting body development. Furthermore, the expression of both fdgA and sasA was partially dependent on the C-signal. These findings expand our understanding of the signal transduction pathway mediated by FruA during development in M. xanthus.

Links

PubMed Online version:10.1074/jbc.M507191200

Keywords

Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Base Sequence; Binding Sites; Biological Transport; DNA/chemistry; Gene Deletion; Gene Library; Genome; Lac Operon; Lipopolysaccharides/chemistry; Molecular Sequence Data; Mutation; Myxococcus xanthus/metabolism; Phenotype; Polysaccharides/chemistry; Protein Binding; Protein Structure, Tertiary; RNA, Messenger/metabolism; Signal Transduction; Transcription Factors/chemistry; Transcription Factors/metabolism; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MYXXD:Q1D7Q3

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0005631: DNAse footprinting evidence used in manual assertion

RefSeq:NC_008095.1

F

Seeded From UniProt

complete

MYXXD:Q1D7Q3

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0005634: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion

RefSeq:NC_008095.1

F

Seeded From UniProt

complete

MYXXD:Q1D7Q3

enables

GO:0001216: DNA-binding transcription activator activity

ECO:0005631: DNAse footprinting evidence used in manual assertion

RefSeq:NC_008095.1

F

Seeded From UniProt

complete

MYXXD:Q1D7Q3

enables

GO:0001216: DNA-binding transcription activator activity

ECO:0005634: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion

RefSeq:NC_008095.1

F

Seeded From UniProt

complete

MYXXD:Q1D7Q3

part_of

GO:0032993: protein-DNA complex

ECO:0005631: DNAse footprinting evidence used in manual assertion

RefSeq:NC_008095.1

C

Seeded From UniProt

complete

MYXXD:Q1D7Q3

part_of

GO:0032993: protein-DNA complex

ECO:0005634: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion

RefSeq:NC_008095.1

C

Seeded From UniProt

complete

Notes

See also

References

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