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PMID:15998810

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Citation

Zhang, Z, Fu, J and Gilmour, DS (2005) CTD-dependent dismantling of the RNA polymerase II elongation complex by the pre-mRNA 3'-end processing factor, Pcf11. Genes Dev. 19:1572-80

Abstract

Pcf11 is one of numerous proteins involved in pre-mRNA 3'-end processing and transcription termination. Using elongation complexes (ECs) formed from purified yeast RNA polymerase II (Pol II), we show that a 140-amino acid polypeptide from yeast Pcf11 is capable of dismantling the EC in vitro. This action depends on the C-terminal domain (CTD) of the largest subunit of Pol II and the CTD-interaction domain (CID) of Pcf11. Our experiments reveal a novel termination mechanism whereby Pcf11 bridges the CTD to the nascent transcript and causes dissociation of both Pol II and the nascent transcript from the DNA in the absence of nucleotide hydrolysis. We posit that conformational changes in the CTD are transduced through Pcf11 to the nascent transcript to cause termination.

Links

PubMed PMC1172063 Online version:10.1101/gad.1296305

Keywords

Base Sequence; Oligonucleotides; RNA Polymerase II/chemistry; RNA Polymerase II/metabolism; RNA Precursors/metabolism; RNA, Messenger/metabolism; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; mRNA Cleavage and Polyadenylation Factors/genetics; mRNA Cleavage and Polyadenylation Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:PCF11

involved_in

GO:0006369: termination of RNA polymerase II transcription

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:PCF11

involved_in

GO:0006369: termination of RNA polymerase II transcription

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:PCF11

enables

GO:0000993: RNA polymerase II complex binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:PCF11

enables

GO:0000993: RNA polymerase II complex binding

ECO:0000353: physical interaction evidence used in manual assertion

SGD:S000002299

F

Seeded From UniProt

complete

YEAST:PCF11

enables

GO:0003729: mRNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:PCF11

enables

GO:0003729: mRNA binding

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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