PMID:15995199

Citation	Suksomtip, M, Liu, P, Anderson, T, Tungpradabkul, S, Wood, DW and Nester, EW (2005) Citrate synthase mutants of Agrobacterium are attenuated in virulence and display reduced vir gene induction. J. Bacteriol. 187:4844-52
Abstract	A citrate synthase (CS) deletion mutant of Agrobacterium tumefaciens C58 is highly attenuated in virulence. The identity of the mutant was initially determined from its amino acid sequence, which is 68% identical to Escherichia coli and 77% identical to Brucella melitensis. The mutant lost all CS enzymatic activity, and a cloned CS gene complemented a CS mutation in Sinorhizobium. The CS mutation resulted in a 10-fold reduction in vir gene expression, which likely accounts for the attenuated virulence. When a plasmid containing a constitutive virG [virG(Con)] locus was introduced into this mutant, the level of vir gene induction was restored to nearly wild-type level. Further, the virG(Con)-complemented CS mutant strain induced tumors that were similar in size and number to those induced by the parental strain. The CS mutation resulted in only a minor reduction in growth rate in a glucose-salts medium. Both the CS mutant and the virG(Con)-complemented CS strain displayed similar growth deficiencies in a glucose-salts medium, indicating that the reduced growth rate of the CS mutant could not be responsible for the attenuated virulence. A search of the genome of A. tumefaciens C58 revealed four proteins, encoded on different replicons, with conserved CS motifs. However, only the locus that when mutated resulted in an attenuated phenotype has CS activity. Mutations in the other three loci did not result in attenuated virulence and any loss of CS activity, and none were able to complement the CS mutation in Sinorhizobium. The function of these loci remains unknown.
Links	PubMed PMC1169492 Online version:10.1128/JB.187.14.4844-4852.2005
Keywords	Agrobacterium tumefaciens/enzymology; Agrobacterium tumefaciens/genetics; Agrobacterium tumefaciens/growth & development; Agrobacterium tumefaciens/pathogenicity; Amino Acid Sequence; Base Sequence; Citrate (si)-Synthase/genetics; Conserved Sequence; DNA Primers; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genome, Bacterial; Peptide Fragments/chemistry; Sequence Alignment; Sequence Homology, Amino Acid; Transcriptional Activation; Virulence/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
AGRT5:A9CJ53		GO:0003878: ATP citrate synthase activity	ECO:0000269:		F		Figure 4 shows shows that the mutant 1392 grew on both plates containing arabinose and kanamycin growth media.Growth in the absence of arabinose indicates that the cell has a functional citrate synthase activity	complete

References

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