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PMID:15988022

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Citation

Okada, T, Sonoda, E, Yoshimura, M, Kawano, Y, Saya, H, Kohzaki, M and Takeda, S (2005) Multiple roles of vertebrate REV genes in DNA repair and recombination. Mol. Cell. Biol. 25:6103-11

Abstract

In yeast, Rev1, Rev3, and Rev7 are involved in translesion synthesis over various kinds of DNA damage and spontaneous and UV-induced mutagenesis. Here, we disrupted Rev1, Rev3, and Rev7 in the chicken B-lymphocyte line DT40. REV1-/- REV3-/- REV7-/- cells showed spontaneous cell death, chromosomal instability/fragility, and hypersensitivity to various genotoxic treatments as observed in each of the single mutants. Surprisingly, the triple-knockout cells showed a suppressed level of sister chromatid exchanges (SCEs), which may reflect postreplication repair events mediated by homologous recombination, while each single mutant showed an elevated SCE level. Furthermore, REV1-/- cells as well as triple mutants showed a decreased level of immunoglobulin gene conversion, suggesting participation of Rev1 in a recombination-based pathway. The present study gives us a new insight into cooperative function of three Rev molecules and the Polzeta (Rev3-Rev7)-independent role of Rev1 in vertebrate cells.

Links

PubMed PMC1168817 Online version:10.1128/MCB.25.14.6103-6111.2005

Keywords

Animals; B-Lymphocytes/enzymology; Cell Line; Chickens/genetics; DNA Damage/genetics; DNA Repair/genetics; DNA-Directed DNA Polymerase/genetics; DNA-Directed DNA Polymerase/physiology; Genes, Immunoglobulin/genetics; Mutagenesis; Mutation; Phenotype; Recombination, Genetic/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:MD2L2

involved_in

GO:0001558: regulation of cell growth

ECO:0000316: genetic interaction evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MD2L2

involved_in

GO:0006302: double-strand break repair

ECO:0000316: genetic interaction evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MD2L2

involved_in

GO:0001558: regulation of cell growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MD2L2

involved_in

GO:0000724: double-strand break repair via homologous recombination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:MD2L2

NOT|involved_in

GO:0000077: DNA damage checkpoint

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:REV1

involved_in

GO:0006302: double-strand break repair

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:REV1

involved_in

GO:0002206: gene conversion of immunoglobulin genes

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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