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PMID:15983

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Citation

Gelfand, DH and Steinberg, RA (1977) Escherichia coli mutants deficient in the aspartate and aromatic amino acid aminotransferases. J. Bacteriol. 130:429-40

Abstract

Two new mutations are described which, together, eliminate essentially all the aminotransferase activity required for de novo biosynthesis of tyrosine, phenylalanine, and aspartic acid in a K-12 strain of Escherichia coli. One mutation, designated tyrB, lies at about 80 min on the E. coli map and inactivates the "tyrosine-repressible" tyrosine/phenylalanine aminotransferase. The second mutation, aspC, maps at about 20 min and inactivates a nonrespressible aspartate aminotransferase that also has activity on the aromatic amino acids. In ilvE- strains, which lack the branched-chain amino acid aminotransferase, the presence of either the tyrosine-repressible aminotransferase or the aspartate aminotransferase is sufficient for growth in the absence of exogenous tyrosine, phenylalanine, or aspartate; the tyrosine-repressible enzyme is also active in leucine biosynthesis. The ilvE gene product alone can reverse a phenylalanine requirement. Biochemical studies on extracts of strains carrying combinations of these aminotransferase mutations confirm the existence of two distinct enzymes with overlapping specificities for the alpha-keto acid analogues of tyrosine, phenylalanine, and aspartate. These enzymes can be distinguished by electrophoretic mobilities, by kinetic parameters using various substrates, and by a difference in tyrosine repressibility. In extracts of an ilvE- tyrB- aspC- triple mutant, no aminotransferase activity for the alpha-keto acids of tyrosine, phenylalanine, or aspartate could be detected.

Links

PubMed PMC235221

Keywords

Aspartate Aminotransferases/metabolism; Aspartic Acid/biosynthesis; Chromosome Mapping; Chromosomes, Bacterial; Coliphages; Escherichia coli/enzymology; Genes; Leucine/biosynthesis; Mutation; Phenylalanine/biosynthesis; Transaminases/metabolism; Transduction, Genetic; Tyrosine/biosynthesis; Tyrosine Transaminase/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:AAT

acts_upstream_of_or_within

GO:0033585: L-phenylalanine biosynthetic process from chorismate via phenylpyruvate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:AAT

acts_upstream_of_or_within

GO:0009094: L-phenylalanine biosynthetic process

ECO:0000316: genetic interaction evidence used in manual assertion

EcoliWiki:ilvE
EcoliWiki:tyrB

P

Seeded From UniProt

complete

ECOLI:TYRB

acts_upstream_of_or_within

GO:0006532: aspartate biosynthetic process

ECO:0000316: genetic interaction evidence used in manual assertion

EcoliWiki:aspC
EcoliWiki:ilvE

P

Seeded From UniProt

complete

ECOLI:ILVE

acts_upstream_of_or_within

GO:0006532: aspartate biosynthetic process

ECO:0000316: genetic interaction evidence used in manual assertion

EcoliWiki:aspC
EcoliWiki:tyrB

P

Seeded From UniProt

complete

ECOLI:AAT

involved_in

GO:0033585: L-phenylalanine biosynthetic process from chorismate via phenylpyruvate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:AAT

enables

GO:0004069: L-aspartate:2-oxoglutarate aminotransferase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:AAT

enables

GO:0004838: L-tyrosine:2-oxoglutarate aminotransferase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:AAT

involved_in

GO:0009094: L-phenylalanine biosynthetic process

ECO:0000316: genetic interaction evidence used in manual assertion

EcoliWiki:ilvE
EcoliWiki:tyrB

P

Seeded From UniProt

complete

ECOLI:TYRB

enables

GO:0004838: L-tyrosine:2-oxoglutarate aminotransferase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:TYRB

involved_in

GO:0006532: aspartate biosynthetic process

ECO:0000316: genetic interaction evidence used in manual assertion

EcoliWiki:aspC
EcoliWiki:ilvE

P

Seeded From UniProt

complete


See also

References

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