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PMID:15978582

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Citation

Burkhardt, C, Müller, M, Badde, A, Garner, CC, Gundelfinger, ED and Püschel, AW (2005) Semaphorin 4B interacts with the post-synaptic density protein PSD-95/SAP90 and is recruited to synapses through a C-terminal PDZ-binding motif. FEBS Lett. 579:3821-8

Abstract

The semaphorins are a large family of proteins that act as guidance signals for axons and dendrites. The class 4 semaphorins are integral membrane proteins that are widely expressed throughout the nervous system. Here, we show that a subclass of these semaphorins is characterized by a PDZ-binding motif at their carboxy-terminus. This sequence mediates the interaction with the post-synaptic density protein PSD-95/SAP90. Co-expression of Sema4B with PSD-95 in COS 7 cells results in the clustering of Sema4B. Sema4B co-localizes with PSD-95 at synaptic contacts between cultured hippocampal neurons. This synaptic localization depends on the presence of the PDZ-binding motif.

Links

PubMed Online version:10.1016/j.febslet.2005.05.079

Keywords

Amino Acid Motifs; Animals; COS Cells; Cercopithecus aethiops; Guanylate Kinase; Hippocampus/cytology; Intracellular Signaling Peptides and Proteins; Membrane Proteins; Mice; Nerve Tissue Proteins/analysis; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurons/metabolism; Protein Structure, Tertiary; Semaphorins/analysis; Semaphorins/chemistry; Semaphorins/metabolism; Synapses/chemistry; Synapses/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:BSN

located_in

GO:0048786: presynaptic active zone

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:O88778

C

  • part_of:(CL:0002608)

Seeded From UniProt

complete

RAT:BSN

located_in

GO:0048786: presynaptic active zone

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:NMDZ1

located_in

GO:0045202: synapse

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P35439

C

  • part_of:(CL:0002608)

Seeded From UniProt

complete

RAT:NMDZ1

located_in

GO:0045202: synapse

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:NRP1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q9Z0G0

F

Seeded From UniProt

complete

MOUSE:DLG4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q9WUH7

F

Seeded From UniProt

complete

MOUSE:DLG4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q64151

F

Seeded From UniProt

complete

MOUSE:DLG4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q62179

F

Seeded From UniProt

complete

MOUSE:DLG4

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:16894)

Seeded From UniProt

complete

MOUSE:SEM4B

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q9Z0G0

F

Seeded From UniProt

complete

MOUSE:SEM4B

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q62108

F

Seeded From UniProt

complete

MOUSE:SEM4B

located_in

GO:0045202: synapse

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SEM4B

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:16894)

Seeded From UniProt

complete

MOUSE:SEM4B

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SEM4C

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q62108

F

Seeded From UniProt

complete

MOUSE:SEM4G

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q62108

F

Seeded From UniProt

complete

MOUSE:GIPC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q62179

F

Seeded From UniProt

complete

MOUSE:GIPC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:P97333

F

Seeded From UniProt

complete

RAT:NMDZ1

part_of

GO:0045202: synapse

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:BSN

part_of

GO:0048786: presynaptic active zone

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:O88778

C

Seeded From UniProt

complete

MOUSE:DLG4

acts_upstream_of_or_within

GO:0035418: protein localization to synapse

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DLG4

part_of

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(EMAPA:16894)

Seeded From UniProt

complete

MOUSE:NMDZ1

part_of

GO:0045202: synapse

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P35439

C

Seeded From UniProt

complete

Notes

See also

References

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