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PMID:15973437

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Citation

Valdez-Taubas, J and Pelham, H (2005) Swf1-dependent palmitoylation of the SNARE Tlg1 prevents its ubiquitination and degradation. EMBO J. 24:2524-32

Abstract

Protein palmitoylation is a post-translational modification that affects a great number of proteins. In most cases, the enzymes responsible for this modification have not been identified. Some proteins use palmitoylation to attach themselves to membranes; however, palmitoylation also occurs in transmembrane proteins, and the function of this palmitoylation is less clear. Here we identify Swf1, a member of the DHHC-CDR family of palmitoyltransferases, as the protein responsible for modifying the yeast SNAREs Snc1, Syn8 and Tlg1, at cysteine residues close to the cytoplasmic end of their single transmembrane domains (TMDs). In an swf1Delta mutant, Tlg1 is mis-sorted to the vacuole. This occurs because unpalmitoylated Tlg1 is recognised by the ubiquitin ligase Tul1, resulting in its targeting to the multivesicular body pathway. Our results suggest that one role of palmitoylation is to protect TMDs from the cellular quality control machinery, and that Swf1 may be the enzyme responsible for most, if not all, TMD-associated palmitoylation in yeast.

Links

PubMed PMC1176453 Online version:10.1038/sj.emboj.7600724

Keywords

Acyltransferases/genetics; Acyltransferases/metabolism; Amino Acid Sequence; Carnitine O-Palmitoyltransferase/metabolism; Endoplasmic Reticulum/metabolism; Genes, Reporter; Humans; Membrane Proteins/antagonists & inhibitors; Membrane Proteins/metabolism; Molecular Sequence Data; Palmitic Acid/metabolism; R-SNARE Proteins; SNARE Proteins; Saccharomyces cerevisiae Proteins/metabolism; Schizosaccharomyces/genetics; Ubiquitin/antagonists & inhibitors; Ubiquitin/metabolism; Ubiquitin-Protein Ligases/metabolism; Vesicular Transport Proteins/antagonists & inhibitors; Vesicular Transport Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:TLG1

located_in

GO:0005802: trans-Golgi network

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SWF1

located_in

GO:0042175: nuclear outer membrane-endoplasmic reticulum membrane network

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SWF1

enables

GO:0016409: palmitoyltransferase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

  • has_input:(SGD:S000000028)|has_input:(SGD:S000000012)|has_input:(SGD:S000002876)

Seeded From UniProt

complete

YEAST:SWF1

involved_in

GO:0018345: protein palmitoylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • has_input:(SGD:S000000028)|has_input:(SGD:S000000012)|has_input:(SGD:S000002876)

Seeded From UniProt

complete

See also

References

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