GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search

Govind, CK, Yoon, S, Qiu, H, Govind, S and Hinnebusch, AG (2005) Simultaneous recruitment of coactivators by Gcn4p stimulates multiple steps of transcription in vivo. Mol. Cell. Biol. 25:5626-38


Transcriptional activation by Gcn4p is dependent on the coactivators SWI/SNF, SAGA, and Srb Mediator, which are recruited by Gcn4p and stimulate assembly of the pre-initiation complex (PIC) at the ARG1 promoter in vivo. We show that recruitment of all three coactivators is nearly simultaneous with binding of Gcn4p at ARG1 and is followed quickly by PIC formation and elongation by RNA polymerase II (Pol II) through the open reading frame. Despite the simultaneous recruitment of coactivators, rapid recruitment of SWI/SNF depends on the histone acetyltransferase (HAT) subunit of SAGA (Gcn5p), a non-HAT function of SAGA, and on Mediator. SAGA recruitment in turn is strongly stimulated by Mediator and the RSC complex. Recruitment of Mediator, by contrast, occurs independently of the other coactivators at ARG1. We confirm the roles of Mediator and SAGA in TATA binding protein (TBP) recruitment and demonstrate that all four coactivators under study enhance Pol II recruitment or promoter clearance following TBP binding. We also present evidence that SWI/SNF and SAGA stimulate transcription elongation downstream from the promoter. These functions can be limited to discrete time intervals, providing evidence for multiple stages in the induction process. Our findings reveal a program of coactivator recruitment and PIC assembly that distinguishes Gcn4p from other yeast activators studied thus far.


PubMed PMC1156971 Online version:10.1128/MCB.25.13.5626-5638.2005


Acetyltransferases/metabolism; Cells, Cultured; Chromatin Immunoprecipitation; DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Gene Expression Regulation, Fungal; Histone Acetyltransferases; Kinetics; Models, Biological; Mutation; Open Reading Frames; Promoter Regions, Genetic; Protein Kinases/chemistry; Protein Kinases/metabolism; Protein Subunits/metabolism; RNA Polymerase II/genetics; RNA Polymerase II/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/chemistry; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Sequence Deletion; TATA-Binding Protein Associated Factors/genetics; TATA-Binding Protein Associated Factors/metabolism; TATA-Box Binding Protein/genetics; TATA-Box Binding Protein/metabolism; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic; Transcriptional Activation



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status



GO:0051123: RNA polymerase II preinitiation complex assembly

ECO:0000315: mutant phenotype evidence used in manual assertion


Seeded From UniProt


See also


See Help:References for how to manage references in GONUTS.