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PMID:15964282

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Citation

Nobile, CJ and Mitchell, AP (2005) Regulation of cell-surface genes and biofilm formation by the C. albicans transcription factor Bcr1p. Curr. Biol. 15:1150-5

Abstract

The impact of many microorganisms on their environment depends upon their ability to form surface bound communities called biofilms [1]. Biofilm formation on implanted medical devices has severe consequences for human health by providing both a portal of entry and a sanctuary for invasive bacterial and fungal pathogens [1 and 2]. Biofilm regulators and adherence molecules are extensively defined for many bacterial pathogens [3, 4, and 5], but not for fungal pathogens such as Candida albicans. Elongated filaments called hyphae are a prominent feature of C. albicans biofilms, and known genes that promote biofilm formation are required for hyphal development [2, 6, 7 and 8]. From a new library of transcription-factor mutants, we identify Bcr1p, a zinc finger protein required for formation of biofilms but not hyphae. Expression analysis shows that Bcr1p activates cell-surface protein and adhesin genes, including several induced during hyphal development. BCR1 expression depends upon the hyphal regulator Tec1p. Thus, BCR1 is a downstream component of the hyphal regulatory network that couples expression of cell-surface genes to hyphal differentiation. Our results indicate that hyphal cells are specialized to present adherence molecules that support biofilm integrity.

Links

PubMed Online version:10.1016/j.cub.2005.05.047

Keywords

Biofilms/growth & development; Candida albicans/genetics; Candida albicans/physiology; Cell Membrane/genetics; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Expression Regulation, Fungal; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Membrane Glycoproteins/genetics; Membrane Glycoproteins/metabolism; Mutation; Transcription Factors/genetics; Transcription Factors/metabolism; Zinc Fingers/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CANAL:TEC1

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

CANAL:TEC1

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CANAL:TEC1

acts_upstream_of_or_within

GO:0044011: single-species biofilm formation on inanimate substrate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CANAL:TEC1

acts_upstream_of_or_within

GO:1900231: regulation of single-species biofilm formation on inanimate substrate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CANAL:BCR1

involved_in

GO:1900231: regulation of single-species biofilm formation on inanimate substrate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CANAL:BCR1

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CANAL:BCR1

involved_in

GO:0044011: single-species biofilm formation on inanimate substrate

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CANAL:BCR1

enables

GO:0003700: DNA-binding transcription factor activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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