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PMID:15950967

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Citation

Couceiro, JR, Martín-Bermudo, MD and Bustelo, XR (2005) Phylogenetic conservation of the regulatory and functional properties of the Vav oncoprotein family. Exp. Cell Res. 308:364-80

Abstract

Vav proteins are phosphorylation-dependent GDP/GTP exchange factors for Rho/Rac GTPases. Despite intense characterization of mammalian Vav proteins both biochemically and genetically, there is little information regarding the conservation of their biological properties in lower organisms. To approach this issue, we have performed a characterization of the regulatory, catalytic, and functional properties of the single Vav family member of Drosophila melanogaster. These analyses have shown that the intramolecular mechanisms controlling the enzyme activity of mammalian Vav proteins are already present in Drosophila, suggesting that such properties have been set up before the divergence between protostomes and deuterostomes during evolution. We also show that Drosophila and mammalian Vav proteins have similar catalytic specificities. As a consequence, Drosophila Vav can trigger oncogenic transformation, morphological change, and enhanced cell motility in mammalian cells. Gain-of-function studies using transgenic flies support the implication of this protein in cytoskeletal-dependent processes such as embryonic dorsal closure, myoblast fusion, tracheal development, and the migration/guidance of different cell types. These results highlight the important roles of Vav proteins in the signal transduction pathways regulating cytoskeletal dynamics. Moreover, they indicate that the foundations for the regulatory and enzymatic activities of this protein family have been set up very early during evolution.

Links

PubMed PMC1447607 Online version:10.1016/j.yexcr.2005.04.035

Keywords

Amino Acid Sequence/physiology; Animals; Animals, Genetically Modified; COS Cells; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Differentiation/physiology; Cell Movement/physiology; Cercopithecus aethiops; Conserved Sequence/physiology; Cytoskeleton/metabolism; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/embryology; Embryo, Nonmammalian/metabolism; Embryonic Development/physiology; Evolution, Molecular; Mice; NIH 3T3 Cells; Organogenesis/physiology; Phylogeny; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/metabolism; Proto-Oncogene Proteins c-vav; Signal Transduction/physiology; rho GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:VAV

involved_in

GO:0016477: cell migration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:VAV

involved_in

GO:0031532: actin cytoskeleton reorganization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:VAV

involved_in

GO:0007520: myoblast fusion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:VAV

involved_in

GO:0007424: open tracheal system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:VAV

involved_in

GO:0007411: axon guidance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:VAV

involved_in

GO:0007391: dorsal closure

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:VAV

enables

GO:0005085: guanyl-nucleotide exchange factor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DROME:VAV

involved_in

GO:0090630: activation of GTPase activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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