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PMID:15936276

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Citation

Patton, A, Knuth, S, Schaheen, B, Dang, H, Greenwald, I and Fares, H (2005) Endocytosis function of a ligand-gated ion channel homolog in Caenorhabditis elegans. Curr. Biol. 15:1045-50

Abstract

Ligand-gated ion channels are transmembrane proteins that respond to a variety of transmitters, including acetylcholine, gamma-aminobutyric acid (GABA), glycine, and glutamate [1 and 2]. These proteins play key roles in neurotransmission and are typically found in the nervous system and at neuromuscular junctions [3]. Recently, acetylcholine receptor family members also have been found in nonneuronal cells, including macrophages [4], keratinocytes [5], bronchial epithelial cells [5], and endothelial cells of arteries [6]. The function of these channels in nonneuronal cells in mammals remains to be elucidated, though it has been shown that the acetylcholine receptor alpha7 subunit is required for acetylcholine-mediated inhibition of tumor necrosis factor release by activated macrophages [4]. We show that cup-4, a gene required for efficient endocytosis of fluids by C. elegans coelomocytes, encodes a protein that is homologous to ligand-gated ion channels, with the highest degree of similarity to nicotinic acetylcholine receptors. Worms lacking CUP-4 have reduced phosphatidylinositol 4,5-bisphosphate levels at the plasma membrane, suggesting that CUP-4 regulates endocytosis through modulation of phospholipase C activity.

Links

PubMed Online version:10.1016/j.cub.2005.04.057

Keywords

Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans/genetics; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/physiology; DNA Primers; DNA, Complementary/genetics; Endocytosis/genetics; Endocytosis/physiology; Green Fluorescent Proteins; Ion Channels/genetics; Ion Channels/physiology; Microscopy, Fluorescence; Molecular Sequence Data; Phosphatidylinositol 4,5-Diphosphate/metabolism; Plasmids/genetics; Receptors, Nicotinic/genetics; Receptors, Nicotinic/physiology; Sequence Alignment; Sequence Analysis, DNA; Type C Phospholipases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:G5ED80

located_in

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CUP4

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CUP4

located_in

GO:0030659: cytoplasmic vesicle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q5FC45

located_in

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q9N4R6

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:Q9N4R6

involved_in

GO:0006897: endocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBRNAi00066346

P

Seeded From UniProt

complete

CAEEL:CUP4

enables

GO:0005216: ion channel activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

CAEEL:CUP4

enables

GO:0015464: acetylcholine receptor activity

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

CAEEL:CUP4

involved_in

GO:0030100: regulation of endocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:CUP4

part_of

GO:0030659: cytoplasmic vesicle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:CUP4

part_of

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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