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PMID:15927177
| Citation |
Kai, T, Williams, D and Spradling, AC (2005) The expression profile of purified Drosophila germline stem cells. Dev. Biol. 283:486-502 |
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| Abstract |
We developed a method to highly purify germline stem cells (GSCs) from the Drosophila ovary, one of the best understood types of adult stem cell. GSCs express variant isoforms of general transcriptional components, translation initiation factors, and several variant ribosomal proteins, including RpL22, a protein enriched in several mammalian stem cells. These novel isoforms may help regulate stem cell gene expression because a reversion assay indicated that at least four were specific for GSCs. By comparative analysis, we identify additional genes enriched in GSCs, including Psc, the Drosophila homolog of the Bmi-1 Polycomb group gene, as well as genes that may delay cytokinesis in pre-meiotic germ cells. By comparing GSCs arrested by BMP over-expression and bam mutation, we hypothesize that mRNA utilization is modulated in differentiating GSC daughters. Our findings suggest that Drosophila and mammalian stem cells utilize at least two regulatory mechanisms in common. |
| Links |
PubMed Online version:10.1016/j.ydbio.2005.04.018 |
| Keywords |
Animals; Cell Differentiation; Cells, Cultured; Cytokinesis/physiology; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; Drosophila/genetics; Drosophila/metabolism; Drosophila Proteins/biosynthesis; Drosophila Proteins/genetics; Energy Metabolism; Female; Gene Expression Profiling; Genotype; Mutation; Oligonucleotide Array Sequence Analysis; Ovary/cytology; Ovary/metabolism; Ovum/cytology; Ovum/metabolism; Protein Biosynthesis; Protein Isoforms/biosynthesis; Protein Isoforms/genetics; RNA-Binding Proteins/biosynthesis; RNA-Binding Proteins/genetics; Ribosomal Proteins/biosynthesis; Ribosomal Proteins/genetics; Stem Cells/cytology; Stem Cells/metabolism; Transcription Factors/biosynthesis; Transcription Factors/genetics |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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