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PMID:15817229

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Citation

Muramoto, T, Takeda, S, Furuya, Y and Urushihara, H (2005) Reverse genetic analyses of gamete-enriched genes revealed a novel regulator of the cAMP signaling pathway in Dictyostelium discoideum. Mech. Dev. 122:733-43

Abstract

Sexual development in Dictyostelium discoideum is initiated by the fusion of opposite mating type cells to form zygote giant cells, which subsequently gather and phagocytose surrounding cells for nutrition to form macrocysts. Here we performed the targeting of 24 highly gamete-enriched genes we previously isolated, and successfully generated knockout mutants for 16 genes and RNAi mutants for 20 genes including 6 genes without disruptants. In the knockout mutants of two genes, cell aggregation toward the giant cells was much less extensive and many cells remained around poorly formed macrocysts. We named these genes tmcB and tmcC. Although macrocyst formation of wild type cells was suppressed by the addition of exogenous cAMP, that of knockout mutants of tmcB was much less sensitive. The mRNA level of phosphodiesterase (pde) was higher and that of its inhibitor (pdi) was lower in the latter cells compared to the parental strains during sexual development. Thus, tmcB appeared to be a novel regulator of the cAMP signaling pathway specific to sexual development. Knockout mutants of tmcC were indistinguishable from the wild type cells with respect to the cAMP response, suggesting that this gene is relevant to other processes.

Links

PubMed Online version:10.1016/j.mod.2004.11.015

Keywords

Amino Acid Sequence; Animals; Animals, Genetically Modified; Cyclic AMP/metabolism; Dictyostelium/metabolism; Dose-Response Relationship, Drug; Genetic Techniques; Intracellular Signaling Peptides and Proteins/genetics; Intracellular Signaling Peptides and Proteins/physiology; Models, Genetic; Molecular Sequence Data; Mutagenesis; Mutation; RNA/metabolism; RNA Interference; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Time Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DICDI:MP05

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:RAC1B

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:FIMB

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q54BC0

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q54C82

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PDI2

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q54EU5

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q54GD5

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q54GM7

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:GMSA

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PSIM

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:ENOA

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q54TI1

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q54W35

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Y9609

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q54X71

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Y3449

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q55BT6

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q55BT7

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:RASZ

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q55CG7

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:TMCB

acts_upstream_of_or_within

GO:0046999: regulation of conjugation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:TMCC

acts_upstream_of_or_within

GO:0046999: regulation of conjugation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:PKS16

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:Q86HI2

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

DICDI:RACF2

acts_upstream_of_or_within

GO:0019953: sexual reproduction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

See also

References

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