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PMID:15792797

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Citation

Johnson, DM, Yamaji, S, Tennant, J, Srai, SK and Sharp, PA (2005) Regulation of divalent metal transporter expression in human intestinal epithelial cells following exposure to non-haem iron. FEBS Lett. 579:1923-9

Abstract

A number of regulatory factors including dietary iron levels can dramatically alter the expression of the intestinal iron transporter DMT1. Here we show that Caco-2 cells exposed to iron for 4h exhibited a significant decrease in plasma membrane DMT1 protein, though total cellular DMT1 levels were unaltered. Following biotinylation of cell surface proteins, there was a significant increase in intracellular biotin-labelled DMT1 in iron-exposed cells. Furthermore, iron-treatment increased levels of DMT1 co-localised with LAMP1, suggesting that the initial response of intestinal epithelial cells to iron involves internalisation and targeting of DMT1 transporter protein towards a late endosomal/lysosomal compartment.

Links

PubMed Online version:10.1016/j.febslet.2005.02.035

Keywords

Biotinylation; Caco-2 Cells; Cation Transport Proteins/analysis; Cation Transport Proteins/genetics; Cation Transport Proteins/metabolism; Cell Membrane/chemistry; Cell Membrane/metabolism; Endosomes/chemistry; Endosomes/metabolism; Humans; Intestinal Mucosa/drug effects; Intestinal Mucosa/metabolism; Intestinal Mucosa/physiology; Iron/metabolism; Iron/pharmacology; Iron-Binding Proteins/analysis; Iron-Binding Proteins/genetics; Iron-Binding Proteins/metabolism; Lysosome-Associated Membrane Glycoproteins; Lysosomes/chemistry; Lysosomes/metabolism; Membrane Glycoproteins/analysis; Membrane Glycoproteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:LAMP1

located_in

GO:0005770: late endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NRAM2

located_in

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NRAM2

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NRAM2

located_in

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NRAM2

located_in

GO:0005770: late endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:EEA1

located_in

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NRAM2

NOT

GO:0010039: response to iron ion

ECO:0000270:

P

P

complete

HUMAN:NRAM2

involved_in

GO:0010039: response to iron ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:NRAM2

NOT|involved_in

GO:0010039: response to iron ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:NRAM2

part_of

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NRAM2

part_of

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NRAM2

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:NRAM2

part_of

GO:0005770: late endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:Q8IUD7

NOT|involved_in

GO:0010039: response to iron ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:Q8IUD7

part_of

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:Q8IUD7

part_of

GO:0016324: apical plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:Q8IUD7

involved_in

GO:0010039: response to iron ion

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:Q8IUD7

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:Q8IUD7

part_of

GO:0005770: late endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:LAMP1

part_of

GO:0005770: late endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:EEA1

part_of

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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