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PMID:15792797
| Citation |
Johnson, DM, Yamaji, S, Tennant, J, Srai, SK and Sharp, PA (2005) Regulation of divalent metal transporter expression in human intestinal epithelial cells following exposure to non-haem iron. FEBS Lett. 579:1923-9 |
|---|---|
| Abstract |
A number of regulatory factors including dietary iron levels can dramatically alter the expression of the intestinal iron transporter DMT1. Here we show that Caco-2 cells exposed to iron for 4h exhibited a significant decrease in plasma membrane DMT1 protein, though total cellular DMT1 levels were unaltered. Following biotinylation of cell surface proteins, there was a significant increase in intracellular biotin-labelled DMT1 in iron-exposed cells. Furthermore, iron-treatment increased levels of DMT1 co-localised with LAMP1, suggesting that the initial response of intestinal epithelial cells to iron involves internalisation and targeting of DMT1 transporter protein towards a late endosomal/lysosomal compartment. |
| Links |
PubMed Online version:10.1016/j.febslet.2005.02.035 |
| Keywords |
Biotinylation; Caco-2 Cells; Cation Transport Proteins/analysis; Cation Transport Proteins/genetics; Cation Transport Proteins/metabolism; Cell Membrane/chemistry; Cell Membrane/metabolism; Endosomes/chemistry; Endosomes/metabolism; Humans; Intestinal Mucosa/drug effects; Intestinal Mucosa/metabolism; Intestinal Mucosa/physiology; Iron/metabolism; Iron/pharmacology; Iron-Binding Proteins/analysis; Iron-Binding Proteins/genetics; Iron-Binding Proteins/metabolism; Lysosome-Associated Membrane Glycoproteins; Lysosomes/chemistry; Lysosomes/metabolism; Membrane Glycoproteins/analysis; Membrane Glycoproteins/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
located_in |
GO:0005770: late endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0005769: early endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0005886: plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0016324: apical plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0005770: late endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0005769: early endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
NOT |
GO:0010039: response to iron ion |
ECO:0000270: |
P |
P |
complete | |||
|
involved_in |
GO:0010039: response to iron ion |
ECO:0000270: expression pattern evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
NOT|involved_in |
GO:0010039: response to iron ion |
ECO:0000270: expression pattern evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005769: early endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016324: apical plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005886: plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005770: late endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
NOT|involved_in |
GO:0010039: response to iron ion |
ECO:0000270: expression pattern evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005769: early endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016324: apical plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010039: response to iron ion |
ECO:0000270: expression pattern evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005886: plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005770: late endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005770: late endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005769: early endosome |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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