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PMID:15772132

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Citation

Thorpe, CJ, Weidinger, G and Moon, RT (2005) Wnt/beta-catenin regulation of the Sp1-related transcription factor sp5l promotes tail development in zebrafish. Development 132:1763-72

Abstract

Tail formation in vertebrates involves the specification of a population of multipotent precursors, the tailbud, which will give rise to all of the posterior structures of the embryo. Wnts are signaling proteins that are candidates for promoting tail outgrowth in zebrafish, although which Wnts are involved, what genes they regulate, and whether Wnts are required for initiation or maintenance steps in tail formation has not been resolved. We show here that both wnt3a and wnt8 are expressed in the zebrafish tailbud and that simultaneous inhibition of both wnt3a and wnt8 using morpholino oligonucleotides can completely block tail formation. In embryos injected with wnt3a and wnt8 morpholinos, expression of genes in undifferentiated presomitic mesoderm is initiated, but not maintained. To identify genes that might function downstream of Wnts in tail formation, a DNA microarray screen was conducted, revealing that sp5l, a member of the Sp1 family of zinc-finger transcription factors, is activated by Wnt signaling. Moreover, we show that sp5l expression in the developing tail is dependent on both wnt3a and wnt8 function. Supporting a role for sp5l in tail formation, we find that inhibition of sp5l strongly enhances the effects of wnt3a inhibition, and overexpression of sp5l RNA is able to completely restore normal tail development in wnt3a morphants. These data place sp5l downstream of wnt3a and wnt8 in a Wnt/beta-catenin signaling pathway that controls tail development in zebrafish.

Links

PubMed Online version:10.1242/dev.01733

Keywords

Animals; Cytoskeletal Proteins/metabolism; Gene Expression Regulation, Developmental; In Situ Hybridization; Intercellular Signaling Peptides and Proteins/metabolism; Mesoderm/metabolism; Oligonucleotide Array Sequence Analysis; Oligonucleotides; Signal Transduction/physiology; Tail/embryology; Tail/metabolism; Trans-Activators/metabolism; Wnt Proteins; Zebrafish/embryology; Zebrafish/metabolism; Zebrafish Proteins/metabolism; beta Catenin

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:F1QK98

involved_in

GO:0001756: somitogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:F1QK98

involved_in

GO:0007498: mesoderm development

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:F1QK98

involved_in

GO:0030903: notochord development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:F1QK98

involved_in

GO:0009953: dorsal/ventral pattern formation

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:F1QK98

involved_in

GO:0009952: anterior/posterior pattern specification

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:F1QK98

involved_in

GO:0036342: post-anal tail morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:F1QK98

involved_in

GO:0036342: post-anal tail morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:Q1JPX0

involved_in

GO:0007498: mesoderm development

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:Q1JPX0

involved_in

GO:0030903: notochord development

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:Q1JPX0

involved_in

GO:0036342: post-anal tail morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:Q6IYD9

involved_in

GO:0001756: somitogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:Q6IYD9

involved_in

GO:0007498: mesoderm development

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:Q6IYD9

involved_in

GO:0030903: notochord development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:Q6IYD9

involved_in

GO:0009953: dorsal/ventral pattern formation

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:Q6IYD9

involved_in

GO:0009952: anterior/posterior pattern specification

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:Q6IYD9

involved_in

GO:0036342: post-anal tail morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:Q6IYD9

involved_in

GO:0036342: post-anal tail morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:Q7T2W1

involved_in

GO:0007498: mesoderm development

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:Q7T2W1

involved_in

GO:0030903: notochord development

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:Q7T2W1

involved_in

GO:0036342: post-anal tail morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0030903: notochord development

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0007498: mesoderm development

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0001756: somitogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0009953: dorsal/ventral pattern formation

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0009952: anterior/posterior pattern specification

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0036342: post-anal tail morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-041217-12

P

Seeded From UniProt

complete

DANRE:WNT8A

involved_in

GO:0036342: post-anal tail morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-050509-2

P

Seeded From UniProt

complete

See also

References

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