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PMID:15743908

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Citation

Crambert, G, Li, C, Claeys, D and Geering, K (2005) FXYD3 (Mat-8), a new regulator of Na,K-ATPase. Mol. Biol. Cell 16:2363-71

Abstract

Four of the seven members of the FXYD protein family have been identified as specific regulators of Na,K-ATPase. In this study, we show that FXYD3, also known as Mat-8, is able to associate with and to modify the transport properties of Na,K-ATPase. In addition to this shared function, FXYD3 displays some uncommon characteristics. First, in contrast to other FXYD proteins, which were shown to be type I membrane proteins, FXYD3 may have a second transmembrane-like domain because of the presence of a noncleavable signal peptide. Second, FXYD3 can associate with Na,K- as well as H,K-ATPases when expressed in Xenopus oocytes. However, in situ (stomach), FXYD3 is associated only with Na,K-ATPase because its expression is restricted to mucous cells in which H,K-ATPase is absent. Coexpressed in Xenopus oocytes, FXYD3 modulates the glycosylation processing of the beta subunit of X,K-ATPase dependent on the presence of the signal peptide. Finally, FXYD3 decreases both the apparent affinity for Na+ and K+ of Na,K-ATPase.

Links

PubMed PMC1087241 Online version:10.1091/mbc.E04-10-0878

Keywords

Amino Acid Sequence; Animals; Cloning, Molecular; Female; Gastric Mucosa/metabolism; Glycosylation; H(+)-K(+)-Exchanging ATPase/chemistry; H(+)-K(+)-Exchanging ATPase/metabolism; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Neoplasm Proteins/chemistry; Neoplasm Proteins/genetics; Neoplasm Proteins/metabolism; Oocytes/metabolism; Protein Processing, Post-Translational; Protein Sorting Signals/genetics; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Sodium-Potassium-Exchanging ATPase/chemistry; Sodium-Potassium-Exchanging ATPase/metabolism; Xenopus laevis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:FXYD3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P12336
UniProtKB:P41181

F

Seeded From UniProt

complete

MOUSE:FXYD3

located_in

GO:0005789: endoplasmic reticulum membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:FXYD3

acts_upstream_of_or_within

GO:0050790: regulation of catalytic activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:FXYD3

enables

GO:0051117: ATPase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P06685
UniProtKB:P07340
UniProtKB:Q63252

F

Seeded From UniProt

complete

MOUSE:FXYD4

enables

GO:0051117: ATPase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P06685
UniProtKB:P07340

F

Seeded From UniProt

complete


See also

References

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