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PMID:15722468

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Citation

Niewiadomski, P, Knappe, S, Geimer, S, Fischer, K, Schulz, B, Unte, US, Rosso, MG, Ache, P, Flügge, UI and Schneider, A (2005) The Arabidopsis plastidic glucose 6-phosphate/phosphate translocator GPT1 is essential for pollen maturation and embryo sac development. Plant Cell 17:760-75

Abstract

Plastids of nongreen tissues can import carbon in the form of glucose 6-phosphate via the glucose 6-phosphate/phosphate translocator (GPT). The Arabidopsis thaliana genome contains two homologous GPT genes, AtGPT1 and AtGPT2. Both proteins show glucose 6-phosphate translocator activity after reconstitution in liposomes, and each of them can rescue the low-starch leaf phenotype of the pgi1 mutant (which lacks plastid phosphoglucoisomerase), indicating that the two proteins are also functional in planta. AtGPT1 transcripts are ubiquitously expressed during plant development, with highest expression in stamens, whereas AtGPT2 expression is restricted to a few tissues, including senescing leaves. Disruption of GPT2 has no obvious effect on growth and development under greenhouse conditions, whereas the mutations gpt1-1 and gpt1-2 are lethal. In both gpt1 lines, distorted segregation ratios, reduced efficiency of transmission in males and females, and inability to complete pollen and ovule development were observed, indicating profound defects in gametogenesis. Embryo sac development is arrested in the gpt1 mutants at a stage before the fusion of the polar nuclei. Mutant pollen development is associated with reduced formation of lipid bodies and small vesicles and the disappearance of dispersed vacuoles, which results in disintegration of the pollen structure. Taken together, our results indicate that GPT1-mediated import of glucose 6-phosphate into nongreen plastids is crucial for gametophyte development. We suggest that loss of GPT1 function results in disruption of the oxidative pentose phosphate cycle, which in turn affects fatty acid biosynthesis.

Links

PubMed PMC1069697 Online version:10.1105/tpc.104.029124

Keywords

Alleles; Antiporters/genetics; Antiporters/metabolism; Arabidopsis/embryology; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Base Sequence; DNA, Plant/genetics; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes, Plant; Genetic Complementation Test; Microscopy, Electron; Monosaccharide Transport Proteins/genetics; Monosaccharide Transport Proteins/metabolism; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Genetically Modified; Plastids/metabolism; Pollen/growth & development; Pollen/ultrastructure

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:GPT2

enables

GO:0005315: inorganic phosphate transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:GPT2

involved_in

GO:0015760: glucose-6-phosphate transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT2

involved_in

GO:0015714: phosphoenolpyruvate transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT2

involved_in

GO:0015713: phosphoglycerate transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT2

enables

GO:0015120: phosphoglycerate transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:GPT2

enables

GO:0015152: glucose-6-phosphate transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:GPT2

enables

GO:0071917: triose-phosphate transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:GPT2

involved_in

GO:0035436: triose phosphate transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT2

NOT|involved_in

GO:0007276: gamete generation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT1

enables

GO:0005315: inorganic phosphate transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:GPT1

involved_in

GO:0015760: glucose-6-phosphate transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT1

involved_in

GO:0015714: phosphoenolpyruvate transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT1

involved_in

GO:0015713: phosphoglycerate transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT1

enables

GO:0015120: phosphoglycerate transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:GPT1

enables

GO:0015152: glucose-6-phosphate transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:GPT1

enables

GO:0071917: triose-phosphate transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:GPT1

involved_in

GO:0035436: triose phosphate transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT1

acts_upstream_of_or_within

GO:0034389: lipid droplet organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT1

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:GPT1

acts_upstream_of_or_within

GO:0007033: vacuole organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT1

acts_upstream_of_or_within

GO:0010152: pollen maturation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:GPT1

located_in

GO:0005777: peroxisome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:GPT1

acts_upstream_of_or_within

GO:0009553: embryo sac development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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