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PMID:15707971

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Citation

Kim, SH, Lee, JY and Kim, J (2005) Characterization of a wide range base-damage-endonuclease activity of mammalian rpS3. Biochem. Biophys. Res. Commun. 328:962-7

Abstract

Mammalian rpS3, a ribosomal protein S3 with a DNA repair endonuclease activity, nicks heavily UV-irradiated DNA and DNA containing AP sites. RpS3 calls for a novel endonucleolytic activity on AP sites generated from pyrimidine dimers by T4 pyrimidine dimer glycosylase activity. This study revealed that rpS3 cleaves the lesions including AP sites, thymine glycols, and other UV damaged lesions such as pyrimidine dimers. This enzyme does not have a glycosylase activity as predicted from its amino acid sequence. However, it has an endonuclease activity on DNA containing thymine glycol, which is exactly overlapped with UV-irradiated or AP DNAs, indicating that rpS3 cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity acting as a base-damage-endonuclease. RpS3 cleaves supercoiled UV damaged DNA more efficiently than the relaxed counterpart, and the endonuclease activity of rpS3 was inhibited by MgCl2 on AP DNA but not on UV-irradiated DNA.

Links

PubMed Online version:10.1016/j.bbrc.2005.01.045

Keywords

DNA/chemistry; DNA/radiation effects; DNA Damage; DNA Repair; DNA Repair Enzymes/chemistry; DNA-(Apurinic or Apyrimidinic Site) Lyase/chemistry; Deoxyribonuclease I/chemistry; Endodeoxyribonucleases/chemistry; Multienzyme Complexes/chemistry; Nucleotides/chemistry; Nucleotides/radiation effects; Ribosomal Proteins/chemistry; Ultraviolet Rays

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:RS3

enables

GO:0003906: DNA-(apurinic or apyrimidinic site) endonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:RS3

enables

GO:0004520: endodeoxyribonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:RS3

enables

GO:0097100: supercoiled DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:RS3

NOT|enables

GO:0019104: DNA N-glycosylase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

MOUSE:RS3

enables

GO:0003906: DNA-(apurinic or apyrimidinic site) endonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:APEX1

enables

GO:0003906: DNA-(apurinic or apyrimidinic site) endonuclease activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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