GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:15673614

From GONUTS
Jump to: navigation, search
Citation

Vertino, AM, Taylor-Jones, JM, Longo, KA, Bearden, ED, Lane, TF, McGehee, RE Jr, MacDougald, OA and Peterson, CA (2005) Wnt10b deficiency promotes coexpression of myogenic and adipogenic programs in myoblasts. Mol. Biol. Cell 16:2039-48

Abstract

Adult myoblasts retain plasticity in developmental potential and can be induced to undergo myogenic, adipogenic, or osteoblastogenic differentiation in vitro. In this report, we show that the balance between myogenic and adipogenic potential in myoblasts is controlled by Wnt signaling. Furthermore, this balance is altered during aging such that aspects of both differentiation programs are coexpressed in myoblasts due to decreased Wnt10b abundance. Mimicking Wnt signaling in aged myoblasts through inhibition of glycogen synthase kinase or through overexpression of Wnt10b resulted in inhibition of adipogenic gene expression and sustained or enhanced myogenic differentiation. On the other hand, myoblasts isolated from Wnt10b null mice showed increased adipogenic potential, likely contributing to excessive lipid accumulation in actively regenerating myofibers in vivo in Wnt10b-/- mice. Whereas Wnt10b deficiency contributed to increased adipogenic potential in myoblasts, the augmented myogenic differentiation potential observed is likely the result of a compensatory increase in Wnt7b during differentiation of Wnt10b-/- myoblasts. No such compensation was apparent in aged myoblasts and in fact, both Wnt5b and Wnt10b were down-regulated. Thus, alteration in Wnt signaling in myoblasts with age may contribute to impaired muscle regenerative capacity and to increased muscle adiposity, both characteristic of aged muscle.

Links

PubMed PMC1073681 Online version:10.1091/mbc.E04-08-0720

Keywords

Adipocytes/cytology; Adipocytes/metabolism; Animals; Cell Differentiation; Cells, Cultured; Gene Expression Regulation; Lipid Metabolism; Mice; Mice, Knockout; Muscle Development; Myoblasts/cytology; Myoblasts/metabolism; Proto-Oncogene Proteins/deficiency; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/metabolism; RNA, Messenger/genetics; RNA, Messenger/metabolism; Wnt Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:FABP4

acts_upstream_of_or_within

GO:0071285: cellular response to lithium ion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:MYOG

acts_upstream_of_or_within

GO:0071285: cellular response to lithium ion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WN10B

acts_upstream_of_or_within

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WN10B

acts_upstream_of_or_within

GO:0014835: myoblast differentiation involved in skeletal muscle regeneration

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3582610

P

  • occurs_in:(EMAPA:35866)
  • results_in_acquisition_of_features_of:(CL:0000056)

Seeded From UniProt

complete

MOUSE:WN10B

acts_upstream_of_or_within

GO:0006629: lipid metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3582610

P

Seeded From UniProt

complete

MOUSE:WN10B

acts_upstream_of_or_within

GO:0048741: skeletal muscle fiber development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3582610

P

  • occurs_in:(EMAPA:35866)
  • results_in_development_of:(CL:0000056)

Seeded From UniProt

complete

MOUSE:WN10B

acts_upstream_of_or_within

GO:0048641: regulation of skeletal muscle tissue development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3582610

P

Seeded From UniProt

complete

MOUSE:WN10B

acts_upstream_of_or_within

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

  • has_input:(MGI:MGI:108061)|has_input:(MGI:MGI:97276)

Seeded From UniProt

complete

MOUSE:WN10B

acts_upstream_of_or_within

GO:0045598: regulation of fat cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3582610

P

Seeded From UniProt

complete

MOUSE:WN10B

acts_upstream_of_or_within

GO:0043403: skeletal muscle tissue regeneration

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:3582610

P

Seeded From UniProt

complete

MOUSE:CTNB1

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000056)

Seeded From UniProt

complete

MOUSE:GSK3A

acts_upstream_of_or_within

GO:0071285: cellular response to lithium ion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:PPARG

acts_upstream_of_or_within

GO:0071285: cellular response to lithium ion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CTNB1

part_of

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0000056)

Seeded From UniProt

complete

MOUSE:CEBPA

acts_upstream_of_or_within

GO:0071285: cellular response to lithium ion

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.